Pawlowski K M, Krol M, Majewska A, Badowska-Kozakiewicz A, Mol J A, Malicka E, Motyl T
Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Poland.
J Physiol Pharmacol. 2009 May;60 Suppl 1:85-94.
Tumor-derived cell lines are widely used as in vitro cancer models. Cell lines historically served as the primary experimental model systems for exploration of tumor cell biology and pharmacology. However, their ability to accurately reflect the phenotype and genotype of the parental histology remains questionable, given the prevalence of documented cell line-specific cytogenetic changes. Sometimes cell line studies are interpreted in the context of artifacts introduced by selection and establishment of cell lines in vitro. This complication has led to difficulties in the extrapolation of biology observed in cell lines to tumor biology in vivo. The aim of our study was to compare gene expression profiles in canine mammary tumor tissue and cell cultures derived from those tumors using cDNA microarrays. Tumors of two different origins were used; chondrosarcoma and adenocarcinoma and their primary cell cultures. It has been found that cell culture gene expression profiles closely resembled those of their corresponding in vivo tumor. In adenocarcinoma and chondrosarcoma only 6.0% and 2.7% of genes respectively, have shown significant difference in expression. In the most cases the difference concerned up-regulation of gene expression in cell lines, particularly genes involved in: protein metabolism and modification, signal transduction and nucleotide, nucleoside and nucleic acid metabolism. These experiments revealed that transcriptome of our primary cell culture corresponds to transcriptome of its parental tumor tissue and for this reason cell culture represents the reliable in vitro model for oncogenomic and pharmacogenomic studies.
肿瘤衍生的细胞系被广泛用作体外癌症模型。细胞系历来是探索肿瘤细胞生物学和药理学的主要实验模型系统。然而，鉴于已记录的细胞系特异性细胞遗传学变化普遍存在，它们准确反映亲代组织学的表型和基因型的能力仍值得怀疑。有时，细胞系研究是在体外选择和建立细胞系所引入的人为因素背景下进行解释的。这种复杂性导致难以将在细胞系中观察到的生物学现象外推至体内肿瘤生物学。我们研究的目的是使用cDNA微阵列比较犬乳腺肿瘤组织及其衍生的细胞培养物中的基因表达谱。使用了两种不同起源的肿瘤；软骨肉瘤和腺癌及其原代细胞培养物。已发现细胞培养基因表达谱与其相应的体内肿瘤的基因表达谱非常相似。在腺癌和软骨肉瘤中，分别只有6.0%和2.7%的基因显示出表达上的显著差异。在大多数情况下，差异涉及细胞系中基因表达的上调，特别是涉及蛋白质代谢和修饰、信号转导以及核苷酸、核苷和核酸代谢的基因。这些实验表明，我们原代细胞培养物的转录组与其亲代肿瘤组织的转录组相对应，因此细胞培养物是肿瘤基因组学和药物基因组学研究的可靠体外模型。
