Institute of Experimental Nuclear Medicine, Shandong University School of Medicine, 44# Wenhua Xi Road, 250012, Jinan, Shandong, China.
Int Urol Nephrol. 2010 Jun;42(2):401-8. doi: 10.1007/s11255-009-9610-5. Epub 2009 Jul 16.
To evaluate the effect of Pygeum africanum on oxidative stress and functional changes of the bladder after diabetes induction.
Thirty-two adult Wistar male rats were treated daily for 8 weeks and grouped as follows: Control group (n = 6), Streptozotocin-induced diabetic group (n = 10), diabetes plus P. africanum group (n = 10), and control plus P. africanum group (n = 6). After diabetes induction for 4 weeks, the diabetes plus P. africanum and control plus P. africanum groups were fed with P. africanum (100 mg/kg, orally) in peanut oil for another 4 weeks. The catalase, superoxide dismutase activity, and malondialdehyde levels were measured as a marker of lipid peroxidation. The levels of inducible nitric oxide synthase were also evaluated. Urodynamic studies were performed to evaluate the functional changes of diabetic bladders after P. africanum treatment.
The catalase and superoxide dismutase activities significantly increased (P < 0.05) and maleic dialdehyde levels significantly decreased from diabetic plus P. africanum group compared with diabetic group (P < 0.05). Immunohistochemical studies showed a significantly decreased number of inducible nitric oxide synthase-positive cells in diabetic plus P. africanum group compared with diabetic group (P < 0.05). In diabetic plus P. africanum group, maximal bladder volume significantly decreased, while bladder pressure and maximal bladder pressure significantly increased compared with diabetic group (P < 0.05).
Early treatment with P. africanum could effectively suppress the oxidative stress status in diabetic bladder and may slow down the process of diabetic cystopathy.
评估非洲臀果木提取物（Pygeum africanum）对糖尿病诱导后膀胱氧化应激和功能变化的影响。
32 只成年雄性 Wistar 大鼠每天接受治疗，持续 8 周，并分为以下几组：对照组（n = 6）、链脲佐菌素诱导的糖尿病组（n = 10）、糖尿病加非洲臀果木提取物组（n = 10）和对照组加非洲臀果木提取物组（n = 6）。在糖尿病诱导 4 周后，糖尿病加非洲臀果木提取物组和对照组加非洲臀果木提取物组开始口服非洲臀果木提取物（100mg/kg），持续 4 周。使用丙二醛水平作为脂质过氧化的标志物来测量过氧化氢酶和超氧化物歧化酶的活性。还评估了诱导型一氧化氮合酶的水平。进行尿动力学研究以评估非洲臀果木提取物治疗后糖尿病膀胱的功能变化。
与糖尿病组相比，糖尿病加非洲臀果木提取物组的过氧化氢酶和超氧化物歧化酶活性显著增加（P < 0.05），丙二醛水平显著降低（P < 0.05）。免疫组织化学研究显示，糖尿病加非洲臀果木提取物组的诱导型一氧化氮合酶阳性细胞数量明显少于糖尿病组（P < 0.05）。与糖尿病组相比，糖尿病加非洲臀果木提取物组的最大膀胱容量显著降低，而膀胱压力和最大膀胱压力显著升高（P < 0.05）。
早期使用非洲臀果木提取物可有效抑制糖尿病膀胱的氧化应激状态，并可能减缓糖尿病膀胱病变的进程。
