Institute for Applied Botany and Pharmacognosy, Department of Farm Animal and Public Health in Veterinary Medicine, University of Veterinary Medicine, Vienna, Austria.
Phytochem Anal. 2009 Sep-Oct;20(5):416-20. doi: 10.1002/pca.1142.
Arbutin is a skin-whitening agent that occurs naturally in the bark and leaves of various plants. It is commonly quantified in plant extracts and skin-whitening products by HPLC.
To develop an alternative gas chromatographic method for the separation and quantification of arbutin in Origanum majorana and Arctostaphylos uva-ursi extracts.
N,O-Bis(trimethylsilyl)acetamide and trimethylchlorosilane were used as silylation reagents, and the gas chromatographic separation of silylated extracts and standards was performed using a DB-5 narrow bore column. GC-MS was used for the compound identification, and the quantification was carried out by GC-FID. The quantitative results were compared with those of HPLC analysis.
The developed method gave a good sensitivity with linearity in the range 0.33-500 mg/mL and recovery >98%, allowing the quantification of arbutin in O. majorana and A. uva-ursi extracts. The relative standard deviations (RSD) relating to intra-day and inter-day precision were <0.002% and <4.8%, respectively. The GC results correlated well with those obtained by HPLC analysis.
The analysis of marjoram and bearberry samples showed that the established GC method was rapid, selective, and demonstrated that arbutin could be screened alternatively by gas chromatography.
熊果苷是一种天然存在于多种植物的树皮和叶子中的皮肤美白剂。它通常通过 HPLC 在植物提取物和美白产品中进行定量。
开发一种替代的气相色谱法，用于分离和定量迷迭香和越桔提取物中的熊果苷。
N,O-双（三甲基硅基）乙酰胺和三甲基氯硅烷被用作硅烷化试剂，并用 DB-5 窄口径柱对硅烷化提取物和标准品进行气相色谱分离。GC-MS 用于化合物鉴定，GC-FID 用于定量。将定量结果与 HPLC 分析进行比较。
所开发的方法具有良好的灵敏度，在线性范围为 0.33-500mg/mL 内，回收率>98%，允许定量测定迷迭香和越桔提取物中的熊果苷。日内和日间精密度的相对标准偏差（RSD）分别<0.002%和<4.8%。GC 结果与 HPLC 分析结果相关性良好。
对马郁兰和熊果样品的分析表明，所建立的 GC 方法快速、选择性强，表明熊果苷可以通过气相色谱法进行替代筛选。
