一种改进的QuEChERS方法结合液相色谱-串联质谱法测定梨皮中熊果苷的方法开发与验证
Development and validation of a modified QuEChERS method coupled with LC-MS/MS to determine arbutin in pear peels.
作者信息
Kim Jueun, Kim Jae Il, Lee Chul Won
机构信息
1Department of Chemistry, Chonnam National University, Gwangju, 61186 Korea.
2Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 61005 Korea.
出版信息
Food Sci Biotechnol. 2016 Aug 31;25(4):987-992. doi: 10.1007/s10068-016-0160-1. eCollection 2016.
A new effective method was developed to determine the concentration of arbutin in pear peels using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The original QuEChERS was modified to enable the extraction of the polar arbutin molecule. Use of an initial 50:50 acetonitrile:water extraction solvent led to the highest extraction efficiency. The arbutin extracted from pear peels was found to be identical to the β-arbutin standard, as confirmed by NMR and LC-MS/MS analyses. For quantitative analysis, the mass spectra of the precursor ion [M+NH] at / 290.0 and the product ion of arbutin at / 163.0 were used. The limit of detection, limit of quantification, linearity, precision, accuracy, and recovery of the proposed method were evaluated. We successfully applied this method to pear samples and it may be suitable for the quantitative analysis of arbutin in other similar plant materials.
开发了一种新的有效方法，使用改良的QuEChERS（快速、简便、廉价、高效、耐用且安全）方法和液相色谱-串联质谱法（LC-MS/MS）来测定梨皮中熊果苷的浓度。对原始的QuEChERS进行了改良，以实现对极性熊果苷分子的提取。使用初始比例为50:50的乙腈:水提取溶剂可获得最高的提取效率。经核磁共振（NMR）和LC-MS/MS分析证实，从梨皮中提取的熊果苷与β-熊果苷标准品相同。进行定量分析时，使用了质荷比（m/z）为290.0的前体离子[M+NH]和熊果苷质荷比为163.0的产物离子的质谱图。对所提出方法的检测限、定量限、线性、精密度、准确度和回收率进行了评估。我们成功地将该方法应用于梨样品，它可能适用于其他类似植物材料中熊果苷的定量分析。
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