Dursunoğlu Benan, Yuca Hafize, Güvenalp Zühal, Gözcü Sefa, Yilmaz Bilal
Atatürk University, Faculty of Pharmacy, Department of Pharmacognosy, Erzurum, Turkey.
Erzincan University, Faculty of Pharmacy, Department of Pharmacognosy, Erzincan, Turkey.
Turk J Pharm Sci. 2018 Dec;15(3):298-303. doi: 10.4274/tjps.38258. Epub 2018 Nov 20.
The aim of our study was to develop a simple, precise, sensitive and specific method for the simultaneous determination of arbutin and hydroquinone in different herbal slimming products using GC-MS.
The methanol and aqueous extracts of nine herbal slimming products in Turkey were evaluated for analysis of arbutin and hydroquinone using GC-MS method.
The retention times of arbutin and hydroquinone were found as 11.32 and 5.44 min, respectively. The linear ranges in this method were 5-500 ng/mL for arbutin and hydroquinone, respectively. The intra- and inter-day precisions, expressed as the relative standard deviation, were less than 1.94 and 2.73%, determined from quality control samples for arbutin and hydroquinone, and accuracy was within 1.13 and 2.56% in terms of relative error, respectively. The limit of detection and quantification were 0.555 and 1.665 ng/mL for arbutin, and 0.031 and 0.093 ng/mL for hydroquinone, respectively.
The developed method can be used for routine quality control analysis of arbutin and hydroquinone in different herbal slimming products.
我们研究的目的是开发一种简单、精确、灵敏且特异的方法，用于使用气相色谱 - 质谱联用仪（GC-MS）同时测定不同草本减肥产品中的熊果苷和对苯二酚。
采用GC-MS法对土耳其9种草本减肥产品的甲醇提取物和水提取物进行熊果苷和对苯二酚分析评估。
熊果苷和对苯二酚的保留时间分别为11.32分钟和5.44分钟。该方法中熊果苷和对苯二酚的线性范围分别为5 - 500 ng/mL。以相对标准偏差表示的日内和日间精密度，由熊果苷和对苯二酚的质量控制样品测定，分别小于1.94%和2.73%，准确度以相对误差计分别在1.13%和2.56%以内。熊果苷的检测限和定量限分别为0.555 ng/mL和1.665 ng/mL，对苯二酚的检测限和定量限分别为0.031 ng/mL和0.093 ng/mL。
所开发的方法可用于不同草本减肥产品中熊果苷和对苯二酚的常规质量控制分析。
