DeBlois R W, Wesley R K
J Virol. 1977 Aug;23(2):227-33. doi: 10.1128/JVI.23.2.227-233.1977.
Viruses above about 60 nm in diameter may be rapidly sized to a few nanometers in their natural hydrated state as they pass one by one through a single pore in a newly developed nanometer-particle analyzer based on the resistive-pulse technique of the Coulter Counter and the use of submicron diameter pores made by the Nuclepore process. Size measurements for several type C oncornaviruses are: Rauscher murine leukemia, 122.3 +/- 2 nm; simian sarcoma, 109.7 +/- 3 nm; Mason-Pfizer monkey, 140.0 +/- 2.5 nm; RD-114, 115 +/- 5 nm; and feline leukemia, 127.4 +/- 2 nm, relative to standard 109-nm latex spheres. The T2 bacteriophage has a volume of (5.10 +/- 0.15) X 10(-16) cm3. Concentrations of viruses near 10(9) to 10(11)/ml that are fairly clear of debris are routinely measurable in a few minutes to an accuracy near 15%. A lower practical count limit is near 5 X 10(7) viruses per ml.
直径约60纳米以上的病毒在其自然水合状态下，当它们逐个通过基于库尔特计数器电阻脉冲技术和使用核孔工艺制造的亚微米直径孔的新开发的纳米颗粒分析仪中的单个孔时，可能会迅速缩小到几纳米。几种C型肿瘤病毒的尺寸测量结果如下：劳舍尔鼠白血病病毒，122.3±2纳米；猿猴肉瘤病毒，109.7±3纳米；梅森 - 辉瑞猴病毒，140.0±2.5纳米；RD - 114病毒，115±5纳米；猫白血病病毒，127.4±2纳米，相对于标准的109纳米乳胶球。T2噬菌体的体积为(5.10±0.15)×10(-16)立方厘米。在几分钟内通常可测量浓度接近10(9)至10(11)/毫升且几乎没有碎片的病毒，精度接近15%。实际计数下限接近每毫升5×10(7)个病毒。
