丙型核糖核酸病毒的生物物理免疫测定法。
Biophysical-immunological assay for ribonucleic acid type C viruses.
作者信息
Olpin J, Oroszlan S, Gilden R V
出版信息
Appl Microbiol. 1974 Jul;28(1):100-5. doi: 10.1128/am.28.1.100-105.1974.
A biophysical and immunological method for characterization of ribonucleic acid type C virus suspensions is described. The method provides a relationship to the total viral mass concentration of the particle titer, the gs antigen titer, and the ultraviolet absorbance (268 nm) of 2% sodium dodecyl sulfate digests. Data for murine, rat, feline, and hamster viruses are shown to be analogous within the test limitations. From these data, an assessment of the viral purity can be made, the structural integrity can be evaluated, an approximate molecular weight can be computed, and the mole ratio of gs antigen can be determined.
本文描述了一种用于表征C型核糖核酸病毒悬液的生物物理和免疫学方法。该方法提供了颗粒滴度、gs抗原滴度与2%十二烷基硫酸钠消化液的紫外线吸光度（268nm）与病毒总质量浓度之间的关系。在测试范围内，小鼠、大鼠、猫和仓鼠病毒的数据显示具有相似性。根据这些数据，可以评估病毒纯度、评估结构完整性、计算近似分子量并确定gs抗原的摩尔比。
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