Amaral André F S, Cymbron Teresa, Gärtner Fátima, Lima Manuela, Rodrigues Armindo S
Spanish National Cancer Research Centre (CNIO), Genetic and Molecular Epidemiology Group, Madrid, Spain.
BMC Vet Res. 2009 Jul 18;5:24. doi: 10.1186/1746-6148-5-24.
Previous studies have provided some evidence of a possible association between cancer and metallothioneins. Whether this relates to an exposure to carcinogenic metals remains unclear.
In order to examine the association between the expression of metallothioneins and bladder tumors, and to compare the levels of arsenic, cadmium, chromium, lead and nickel in animals with bladder tumors and animals without bladder tumors, 37 cases of bovine bladder tumors and 17 controls were collected. The detection and quantification of metallothioneins in bladder tissue of both cases and controls was performed by immunohistochemistry. And the quantification of metals in tissue and hair was assessed by inductively coupled plasma - mass spectrometry.
Increased expression of metallothioneins was associated with bladder tumors when compared with non-tumoral bladder tissue (OR = 9.3, 95% CI: 1.0 - 480). The concentrations of cadmium, chromium, lead and nickel in hair of cases were significantly higher than those of controls. However, as for the concentration of metals in bladder tissue, the differences were not significant.
Though the sample size was small, the present study shows an association between bladder tumors and metallothioneins. Moreover, it shows that concentrations of metals such as cadmium, chromium, lead and nickel in hair may be used as a biomarker of exposure.
先前的研究已提供了一些癌症与金属硫蛋白之间可能存在关联的证据。这是否与接触致癌金属有关仍不清楚。
为了研究金属硫蛋白的表达与膀胱肿瘤之间的关联，并比较患有膀胱肿瘤的动物和未患膀胱肿瘤的动物体内砷、镉、铬、铅和镍的含量，收集了37例牛膀胱肿瘤病例和17例对照。通过免疫组织化学对病例组和对照组膀胱组织中的金属硫蛋白进行检测和定量。并通过电感耦合等离子体质谱法评估组织和毛发中的金属含量。
与非肿瘤性膀胱组织相比，金属硫蛋白表达增加与膀胱肿瘤有关（比值比=9.3，95%置信区间：1.0-480）。病例组毛发中镉、铬、铅和镍的浓度显著高于对照组。然而，膀胱组织中金属的浓度差异不显著。
尽管样本量较小，但本研究显示膀胱肿瘤与金属硫蛋白之间存在关联。此外，研究表明毛发中镉、铬、铅和镍等金属的浓度可作为接触的生物标志物。
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