Hu Kaifeng, Doucleff Michaeleen, Clore G Marius
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, USA.
J Magn Reson. 2009 Oct;200(2):173-7. doi: 10.1016/j.jmr.2009.06.019. Epub 2009 Jun 27.
We present a new version of the 3D TROSY HNCO pulse scheme, referred to as HR-TROSY HNCO, with comparable resolution in the (15)N dimension to a 2D (1)H-(15)N HSQC experiment. In the conventional 3D TROSY HNCO, the constant time period (1/2J(NC) approximately 32 ms) severely limits the maximum resolution in the (15)N dimension. In the HR-TROSY HNCO experiment presented here, both constant time periods (approximately 32 ms each) for coherence forward and backward transfer between (15)N and (13)C' are utilized to double the (15)N evolution time. This leads to a dramatic enhancement in peak separation along the (15)N dimension, making the HR-TROSY HNCO an ideal pulse scheme for accurate paramagnetic relaxation enhancement and residual dipolar coupling measurements.
我们展示了一种新的3D TROSY HNCO脉冲序列，称为HR-TROSY HNCO，其在（15）N维度上的分辨率与二维（1）H-（15）N HSQC实验相当。在传统的3D TROSY HNCO中，恒定时间周期（1/2J（NC）约32毫秒）严重限制了（15）N维度上的最大分辨率。在此展示的HR-TROSY HNCO实验中，用于（15）N和（13）C'之间相干向前和向后转移的两个恒定时间周期（每个约32毫秒）被用来使（15）N演化时间加倍。这导致沿（15）N维度的峰分离显著增强，使HR-TROSY HNCO成为用于精确顺磁弛豫增强和残余偶极耦合测量的理想脉冲序列。
