Freitas R M
Laboratory of Physiology and Pharmacology, Federal University of Piaui, Piaui, Brazil.
Neurosci Lett. 2009 Oct 25;462(3):225-9. doi: 10.1016/j.neulet.2009.07.037. Epub 2009 Jul 16.
The relationship between free radical and scavenger enzymes has been found in the epileptic phenomena and reactive oxygen species have been implicated in seizure-induced neurodegeneration. Using the epilepsy model obtained by systemic administration of pilocarpine in rats, we investigated the lipid peroxidation, nitrite content, superoxide dismutase (SOD) and catalase activities in the hippocampus of rats during chronic period. The enzyme activities as well as the lipid peroxidation and nitrite concentrations were measured using spectrophotometric methods and the results compared to values obtained from saline-treated animals. The superoxide dismutase and catalase activities increased during the chronic phase. In addition, lipid peroxidation and nitrite levels increased in same period in the hippocampus of animals observed during spontaneous recurrent seizures. Previous studies showed that animals presenting seizures and submitted to 24h of status epilepticus showed normal levels of superoxide dismutase and increased in catalase activities as well as an increase in hippocampal lipid peroxidation and nitrite concentrations. These results show a direct evidence of lipid peroxidation and nitrite during seizure activity that could be responsible for neuronal damage in the hippocampus of rats, during the establishment of pilocarpine model of epilepsy.
在癫痫现象中已发现自由基与清除酶之间的关系，并且活性氧已被认为与癫痫发作诱导的神经退行性变有关。利用通过对大鼠全身注射毛果芸香碱获得的癫痫模型，我们研究了慢性期大鼠海马中的脂质过氧化、亚硝酸盐含量、超氧化物歧化酶（SOD）和过氧化氢酶活性。使用分光光度法测量酶活性以及脂质过氧化和亚硝酸盐浓度，并将结果与盐水处理动物获得的值进行比较。在慢性期，超氧化物歧化酶和过氧化氢酶活性增加。此外，在观察到自发反复癫痫发作的动物海马中，同一时期脂质过氧化和亚硝酸盐水平增加。先前的研究表明，出现癫痫发作并经历24小时癫痫持续状态的动物超氧化物歧化酶水平正常，过氧化氢酶活性增加，同时海马脂质过氧化和亚硝酸盐浓度也增加。这些结果直接证明了在癫痫活动期间脂质过氧化和亚硝酸盐的存在，这可能是在毛果芸香碱癫痫模型建立过程中大鼠海马神经元损伤的原因。
