一种基于罗丹明B修饰的β-环糊精检测HeLa细胞低pH区域的高灵敏度探针。
A highly sensitive probe detecting low pH area of HeLa cells based on rhodamine B modified beta-cyclodextrins.
作者信息
Hasegawa Takuto, Kondo Yoshihiko, Koizumi Yukio, Sugiyama Toshihiro, Takeda Akane, Ito Shinichi, Hamada Fumio
机构信息
Department of Life Science, Akita University, Tegata, Japan.
出版信息
Bioorg Med Chem. 2009 Aug 15;17(16):6015-9. doi: 10.1016/j.bmc.2009.06.046. Epub 2009 Jun 27.
Two kinds of rhodamine modified beta-cyclodextrins (R-1 and R-2), which are coupled up ethylene diamine (EDA) and tetraethylene pentamine (TEPA) between Rh B and beta-cyclodextrin, respectively, have been synthesized. R-1 and 2 work as a new fluorogenic probe for monitoring pH of Hela cells, and MTT of assay R-1, R-2, and rhodamine B indicate that less a cytotoxicity of those R-1 and R-2 than that of rhodamine B, where R-1 has much less one than that of R-2. The fluorogenetic probe capability of R-2 was recognized in an area of acidic area in living cell, which is lysosome.
已合成了两种罗丹明修饰的β-环糊精（R-1和R-2），它们分别是在罗丹明B和β-环糊精之间偶联了乙二胺（EDA）和四乙烯五胺（TEPA）。R-1和R-2作为监测Hela细胞pH值的新型荧光探针，R-1、R-2和罗丹明B的MTT测定表明，R-1和R-2的细胞毒性比罗丹明B小，其中R-1的细胞毒性比R-2小得多。R-2的荧光探针能力在活细胞的酸性区域即溶酶体中得到了证实。
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