Department of Chemical Biology, College of Chemistry and Chemical Engineering, and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, China.
Analyst. 2011 Sep 21;136(18):3698-706. doi: 10.1039/c1an15108h. Epub 2011 Jul 27.
The parameters of intracellular acidic compartments are altered in apoptosis, autophagy, cancer metastasis, etc. Low background and selective staining of lysosomes and autophagosomes was achieved with N-(rhodamine 6G)-lactam-ethylenediamine (R6G-EDA) which fluoresces in acidic vesicles via pH mediated ring opening of the intramolecular lactam. Long retention time of R6G-EDA in lysosomes and its exceptional stability against photo-bleaching allow full time tracking of lysosome morphology changes in tumor necrosis factor-α (TNF-α) triggered cell death, suggesting its utility for acidic vesicle studies in cell biology.
细胞内酸性隔室的参数在细胞凋亡、自噬、癌症转移等过程中发生改变。N-（若丹明 6G）-内酰胺-乙二胺（R6G-EDA）可通过分子内内酰胺的 pH 介导开环在酸性囊泡中发出荧光，从而实现溶酶体和自噬体的低背景和选择性染色。R6G-EDA 在溶酶体中的保留时间长，对光漂白的稳定性异常高，允许对肿瘤坏死因子-α（TNF-α）触发的细胞死亡中溶酶体形态变化进行全时跟踪，表明其可用于细胞生物学中的酸性囊泡研究。
