Bailey-Serres J, Sorenson Reed, Juntawong Piyada
Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California, Riverside, CA 92521-0124, USA.
Trends Plant Sci. 2009 Aug;14(8):443-53. doi: 10.1016/j.tplants.2009.05.004. Epub 2009 Jul 18.
mRNA-ribonucleoprotein (mRNP) complexes mediate post-transcriptional control mechanisms in the cell nucleus and cytoplasm. Transcriptional control is paramount to gene expression but is followed by regulated nuclear pre-mRNA maturation and quality control processes that culminate in the export of a functional transcript to the cytoplasm. Once in the cytosol, mRNPs determine the activity of individual mRNAs through regulation of localization, translation, sequestration and turnover. Here, we review how quantitative assessment of mRNAs in distinct cytoplasmic mRNPs, such as polyribosomes (polysomes), has provided new perspectives on post-transcriptional regulation from the global to gene-specific level. In addition, we explore recent genetic and biochemical studies of cytoplasmic mRNPs that have begun to expose RNA-binding proteins in an integrated network that fine-tunes gene expression.
信使核糖核酸-核糖核蛋白（mRNP）复合物介导细胞核和细胞质中的转录后调控机制。转录调控对于基因表达至关重要，但随后会进行受调控的核前体信使核糖核酸成熟和质量控制过程，最终将功能性转录本输出到细胞质中。一旦进入细胞质溶胶，mRNP通过调控定位、翻译、隔离和周转来决定单个信使核糖核酸的活性。在这里，我们回顾了如何对不同细胞质mRNP（如多核糖体）中的信使核糖核酸进行定量评估，从而为从全局到基因特异性水平的转录后调控提供了新的视角。此外，我们还探讨了最近对细胞质mRNP的遗传学和生物化学研究，这些研究已开始揭示一个精细调节基因表达的整合网络中的RNA结合蛋白。
