Coria-Galindo Elsa, Rangel-Huerta Emma, Verdugo-Rodríguez Antonio, Brousset Dulce, Salazar Sandie, Padilla-Noriega Luis
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, DF, Mexico.
J Wildl Dis. 2009 Jul;45(3):722-8. doi: 10.7589/0090-3558-45.3.722.
Group A rotaviruses infect and cause diarrhea in the young of a broad range of terrestrial mammals, but it is unknown, to our knowledge, whether they infect marine mammals. During February and March of 2002 and 2003, we collected 125 serum samples and 18 rectal swab samples from Galapagos sea lion pups (GSL, Zalophus wollebaeki), and 22 serum samples from Galapagos fur seal pups (GFS, Arctocephalus galapagoensis) from nine islands of the Galapagos archipelago, Ecuador. Sera were tested for antibodies (immunoglobulin G [IgG]) to rotavirus by an enzyme immunoassay using rhesus rotavirus as the capture antigen. In addition, rectal swabs were analyzed for the presence of rotavirus genomic double-stranded RNA by silver-stained polyacrylamide gel electrophoresis. Antibodies to rotavirus were detected in 27 GSL pups (22%) and five GFS pups (23%), and rotavirus RNA was detected in the fecal sample from one GSL pup (6%). These results provide the first evidence that rotavirus infections are prevalent at an early age in Galapagos sea lions and Galapagos fur seals.
A组轮状病毒可感染多种陆生哺乳动物幼崽并导致腹泻，但据我们所知，它们是否感染海洋哺乳动物尚不清楚。在2002年2月和3月以及2003年2月和3月期间，我们从厄瓜多尔加拉帕戈斯群岛九个岛屿的加拉帕戈斯海狮幼崽（GSL，Zalophus wollebaeki）身上采集了125份血清样本和18份直肠拭子样本，并从加拉帕戈斯毛皮海狮幼崽（GFS，Arctocephalus galapagoensis）身上采集了22份血清样本。通过使用恒河猴轮状病毒作为捕获抗原的酶免疫测定法检测血清中针对轮状病毒的抗体（免疫球蛋白G [IgG]）。此外，通过银染聚丙烯酰胺凝胶电泳分析直肠拭子中是否存在轮状病毒基因组双链RNA。在27只加拉帕戈斯海狮幼崽（22%）和5只加拉帕戈斯毛皮海狮幼崽（23%）中检测到了针对轮状病毒的抗体，并且在一只加拉帕戈斯海狮幼崽的粪便样本中检测到了轮状病毒RNA（6%）。这些结果提供了首个证据，证明轮状病毒感染在加拉帕戈斯海狮和加拉帕戈斯毛皮海狮幼崽中很普遍。
