Nynca A, Jablonska O, Slomczynska M, Petroff B K, Ciereszko R E
Department of Animal Physiology, University of Warmia and Mazury, Olsztyn, Poland.
J Physiol Pharmacol. 2009 Jun;60(2):95-105.
The aims of the study were to compare the in vitro effects of daidzein or 17beta-estradiol (E(2)) on: 1) progesterone (P(4)) secretion by luteinized granulosa cells harvested from large porcine follicles, as well as 2) estrogen receptor alpha and beta (ERalpha and ERbeta) mRNA and protein expression in the cells. In addition, the effect of daidzein on E(2) secretion and viability of the granulosa cells was examined. We found that basal and gonadotropin-stimulated P(4) secretion were inhibited in granulosa cells cultured in the presence of daidzein either for 24 or 48 hours. In contrast to daidzein, E(2) reduced P(4) secretion only during 24-hour cell cultures increasing it during longer cultures. Daidzein did not affect E(2) secretion by granulosa cells. The expression of ERalpha and ERbeta mRNA, as well as ERbeta protein, was up-regulated by daidzein but unaffected by E(2). To conclude, the soy estrogen daidzein acts directly on the porcine ovary to decrease progesterone production and to increase expression of ERbeta mRNA and protein. Daidzein actions in porcine luteinized granulosa cells differ from those of estradiol and it may suggest disadvantageous effects of the phytoestrogen on reproductive processes in females.
本研究的目的是比较大豆苷元或17β-雌二醇（E₂）对以下方面的体外作用：1）从大型猪卵泡中收获的黄体化颗粒细胞分泌孕酮（P₄），以及2）细胞中雌激素受体α和β（ERα和ERβ）的mRNA和蛋白表达。此外，还检测了大豆苷元对颗粒细胞E₂分泌和活力的影响。我们发现，在存在大豆苷元的情况下培养24或48小时的颗粒细胞中，基础和促性腺激素刺激的P₄分泌均受到抑制。与大豆苷元不同，E₂仅在24小时细胞培养期间降低P₄分泌，而在更长时间培养时则增加P₄分泌。大豆苷元不影响颗粒细胞的E₂分泌。大豆苷元上调了ERα和ERβ的mRNA以及ERβ蛋白的表达，但E₂对其无影响。总之，大豆雌激素大豆苷元直接作用于猪卵巢，降低孕酮生成并增加ERβ mRNA和蛋白的表达。大豆苷元在猪黄体化颗粒细胞中的作用与雌二醇不同，这可能表明这种植物雌激素对雌性生殖过程有不利影响。
