Islam-Faridi M N, Nelson C D, DiFazio S P, Gunter L E, Tuskan G A
Southern Institute of Forest Genetics, Southern Research Station, U.S. Forest Service, Forest Tree Molecular Cytogenetics Laboratory, Department of Ecosystem Science and Management, Texas A&M University, College Station, TX, USA.
Cytogenet Genome Res. 2009;125(1):74-80. doi: 10.1159/000218749. Epub 2009 Jul 14.
The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 18S-28S rDNA sites and one 5S rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.
利用荧光原位杂交（FISH）技术对毛果杨中的18S - 28S rDNA和5S rDNA基因座进行了定位。鉴定出两个18S - 28S rDNA位点和一个5S rDNA位点，它们位于3条不同染色体的末端。在每条染色体的远端观察到来自拟南芥型端粒重复序列的FISH信号。从基于基因组序列组装的2个连锁群（LG-I和LG-VI）中选择的6个BAC克隆如预期一样定位在2条染色体上。来自LG-I的BAC与该互补组中最长的染色体杂交。发现所有BAC的位置与序列组装位置一致。BAC-FISH将有助于描绘毛果杨的每条染色体，并改进这种模式被子植物树种的序列组装。
