Yan Wei-Hua, Lin Aifen, Chen Bao-Guo, Chen Shi-Yong
Medical Research Center, Taizhou Hospital of Zhejiang Province, Wenzhou Medical College, Linhai, Zhejiang, People's Republic of China.
J Infect Dis. 2009 Sep 1;200(5):820-6. doi: 10.1086/604733.
Alteration of HLA expression or cytokine production plays a crucial role in the pathogenesis of human cytomegalovirus (HCMV) infection. HLA-G has been suggested to be involved in HCMV infection, and modulation of HLA-G expression by interferon (IFN)-gamma and interleukin (IL)-10 has been reported. However, the clinical relevance of HLA-G in HCMV infection remains unknown.
The study included 75 patients with active HCMV infection (age range, 1-4.5 years) and 150 sex- and age-matched healthy control subjects (age range, 1-5 years). HLA-G expression in peripheral monocytes from patients (n=38) and control subjects (n=20) was analyzed using flow cytometry. Plasma levels of soluble HLA-G (in 75 patients and 150 control subjects), IL-10 (in 75 patients and 40 control subjects), and IFN-gamma (in 75 patients and 40 control subjects) were determined using enzyme-linked immunosorbent assay.
The mean percentage of HLA-G-positive monocytes among patients with active HCMV infection was dramatically increased, compared with that among healthy control subjects (6.33% vs 1.64%; P<.001). Similarly, significant increases were observed in soluble HLA-G level (median, 54.91 vs 21.32 U/mL; P<.001) and IL-10 level (median, 9.24 vs 1.82 ng/mL; P<.001). Although the expression of IFN-gamma was higher in patients with active HCMV infection than in healthy control subjects, the difference was not statistically significant (median, 1254.46 vs 887.05 ng/mL; P=.070). Furthermore, no correlation was established between HLA-G expression and levels of IL-10 or IFN-gamma.
HLA-G expression in monocytes and plasma soluble HLA-G and IL-10 levels were increased during active HCMV infection.
人类巨细胞病毒（HCMV）感染发病机制中，人类白细胞抗原（HLA）表达改变或细胞因子产生起着关键作用。有研究表明HLA - G参与HCMV感染，且已有报告称干扰素（IFN）-γ和白细胞介素（IL）-10可调节HLA - G表达。然而，HLA - G在HCMV感染中的临床相关性仍不明确。
该研究纳入75例活动性HCMV感染患者（年龄范围1 - 4.5岁）和150例性别及年龄匹配的健康对照者（年龄范围1 - 5岁）。采用流式细胞术分析患者（n = 38）和对照者（n = 20）外周血单核细胞中HLA - G的表达。使用酶联免疫吸附测定法测定75例患者和150例对照者血浆中可溶性HLA - G水平、75例患者和40例对照者血浆中IL - 10水平以及75例患者和40例对照者血浆中IFN -γ水平。
与健康对照者相比，活动性HCMV感染患者中HLA - G阳性单核细胞的平均百分比显著升高（6.33%对1.64%；P <.001）。同样，可溶性HLA - G水平（中位数54.91对21.32 U/mL；P <.001）和IL - 10水平（中位数9.24对1.82 ng/mL；P <.001）也显著升高。尽管活动性HCMV感染患者中IFN -γ的表达高于健康对照者，但差异无统计学意义（中位数1254.46对887.05 ng/mL；P = 0.070）。此外，未发现HLA - G表达与IL - 10或IFN -γ水平之间存在相关性。
活动性HCMV感染期间，单核细胞中HLA - G表达以及血浆中可溶性HLA - G和IL - 10水平升高。
