Human Tissue Bank, Taizhou Hospital of Zhejiang Province, Wenzhou Medical College, Linhai, Zhejiang, China.
Hum Immunol. 2011 Feb;72(2):159-65. doi: 10.1016/j.humimm.2010.11.009. Epub 2010 Nov 16.
A novel H1N1 virus of swine origin (H1N1v) recently caused a pandemic; however, knowledge of immunologic aspects of the virus infection are limited. Human leukocyte antigen-G (HLA-G) was speculated to play critical roles in viral infection, although its clinical relevance in H1N1 infection remains unknown. In this study, HLA-G expression in peripheral T lymphocytes, monocytes, and CD4(+) CD25(+) FoxP3+ regulatory T (Treg) cells (in 50 H1N1v-infected and 41 seasonal H1N1-infected patients and 27 control subjects) were analyzed by flow cytometry. Plasma-soluble HLA-G (sHLA-G, in 28 H1N1v-infected, 29 seasonal H1N1-infected patients and 85 control subjects) were determined with enzyme-linked immunosorbent assay. The percentage of HLA-G-positive T lymphocytes and monocytes among patients with H1N1v and seasonal H1N1 infections was dramatically increased compared with controls (all p < 0.001). Treg was markedly increased among H1N1v- infected patients compared with normal controls (p = 0.041), but not for the seasonal H1N1-infected patients. Meanwhile, no significant difference was observed for sHLA-G levels between the groups. Together, cell surface HLA-G expression was markedly induced in H1N1v-infected and seasonal H1N1-infected patients, and increased Treg was observed only in H1N1v-infected patients. Given its immune-suppressive property, elevated cell surface HLA-G expression may help to explain the virus escaping from host immune responses.
一种新型的猪源 H1N1 病毒（H1N1v）最近引发了一场大流行；然而，人们对该病毒感染的免疫学方面的了解有限。人类白细胞抗原-G（HLA-G）被推测在病毒感染中发挥关键作用，尽管其在 H1N1 感染中的临床相关性尚不清楚。在这项研究中，通过流式细胞术分析了外周 T 淋巴细胞、单核细胞和 CD4+CD25+FoxP3+调节性 T（Treg）细胞中的 HLA-G 表达（在 50 例 H1N1v 感染和 41 例季节性 H1N1 感染患者以及 27 例对照者中）。采用酶联免疫吸附试验测定血浆可溶性 HLA-G（sHLA-G，在 28 例 H1N1v 感染、29 例季节性 H1N1 感染患者和 85 例对照者中）。与对照组相比，H1N1v 和季节性 H1N1 感染患者的 HLA-G 阳性 T 淋巴细胞和单核细胞的百分比明显增加（均 p<0.001）。与正常对照组相比，H1N1v 感染患者的 Treg 明显增加（p=0.041），但季节性 H1N1 感染患者则没有。同时，各组间 sHLA-G 水平无显著差异。总之，H1N1v 感染和季节性 H1N1 感染患者的细胞表面 HLA-G 表达明显增加，而仅在 H1N1v 感染患者中观察到 Treg 增加。鉴于其免疫抑制特性，细胞表面 HLA-G 表达的升高可能有助于解释病毒逃避宿主免疫反应的现象。
