Cancer Metastasis Research Center, Yonsei University College of Medicine, Republic of Korea.
Cancer Lett. 2010 Feb 1;288(1):124-32. doi: 10.1016/j.canlet.2009.06.031. Epub 2009 Jul 19.
It has been suggested that vasculogenesis by endothelial progenitor cells (EPC) as well as angiogenesis play an important role in the production of blood vessels in neoplasm. The present study was designed to isolate and characterize the EPC in gastric cancer patients as a tumor specific angiogenesis marker. The cells derived from CD34 positive PBMC presented with a cobblestone appearance at 28 days, revealing differentiation into endothelial cells. They were also positive to the LDL-uptake reaction, showing that they have biological endothelial cell functions. These cells demonstrated tube formation, showing their ability to participate in neovascularization. The cells derived from CD34 positive PBMC expressed CD133 and demonstrated telomerase activity, showing the stem cell character. In xenograft model, EPC derived from CD34 positive PBMC mobilized mainly into tumor area after being injected through tail vein. With isolation, ex vivo amplification and characterization of EPC from gastric cancer patients receiving chemotherapy, endothelial progenitor cells may be used as a candidate prognostic and predictive biomarker for cancer.
已经有人提出，内皮祖细胞（EPC）的血管生成以及血管生成在肿瘤中血管的产生中起着重要作用。本研究旨在分离和鉴定胃癌患者的 EPC，作为肿瘤特异性血管生成标志物。从 CD34 阳性 PBMC 中分离出来的细胞在第 28 天呈现出鹅卵石外观，表明分化为内皮细胞。它们还对 LDL 摄取反应呈阳性，表明它们具有生物学内皮细胞功能。这些细胞形成管状结构，显示出它们参与新血管生成的能力。从 CD34 阳性 PBMC 中分离出来的细胞表达 CD133 并表现出端粒酶活性，显示出干细胞特征。在异种移植模型中，EPC 从 CD34 阳性 PBMC 中分离出来，在通过尾静脉注射后主要迁移到肿瘤区域。通过对接受化疗的胃癌患者的 EPC 进行分离、体外扩增和鉴定，内皮祖细胞可用作癌症预后和预测的候选生物标志物。
