Küpfer Mara, Kuhnert Peter, Korczak Bożena M, Peduzzi Raffaele, Demarta Antonella
Cantonal Institute of Microbiology, Microbial Ecology, CH-6500 Bellinzona, Switzerland.
Institute of Veterinary Bacteriology, University of Bern, CH-3001 Bern, Switzerland.
Int J Syst Evol Microbiol. 2006 Dec;56(Pt 12):2743-2751. doi: 10.1099/ijs.0.63650-0.
Genetic relationships among bacterial strains belonging to the genus Aeromonas were inferred from 16S rRNA, gyrB and rpoB gene sequences. Twenty-eight type or collection strains of the recognized species or subspecies and 33 Aeromonas strains isolated from human and animal specimens as well as from environmental samples were included in the study. As reported previously, the 16S rRNA gene sequence is highly conserved within the genus Aeromonas, having only limited resolution for this very tight group of species. Analysis of a 1.1 kb gyrB sequence confirmed that this gene has high resolving power, with maximal interspecies divergence of 15.2 %. Similar results were obtained by sequencing only 517 bp of the rpoB gene, which showed maximal interspecies divergence of 13 %. The topologies of the gyrB- and rpoB-derived trees were similar. The results confirm the close relationship of species within the genus Aeromonas and show that a phylogenetic approach including several genes is suitable for improving the complicated taxonomy of the genus.
通过16S rRNA、gyrB和rpoB基因序列推断了气单胞菌属细菌菌株之间的遗传关系。本研究纳入了28株已确认物种或亚种的模式菌株或保藏菌株，以及33株从人类和动物标本以及环境样本中分离出的气单胞菌菌株。如先前报道，16S rRNA基因序列在气单胞菌属内高度保守，对于这一紧密的物种群分辨率有限。对1.1 kb的gyrB序列分析证实，该基因具有高分辨率，种间最大差异为15.2%。仅对rpoB基因的517 bp进行测序也得到了类似结果，种间最大差异为13%。gyrB基因和rpoB基因推导的树的拓扑结构相似。结果证实了气单胞菌属内各物种之间的密切关系，并表明包括多个基因的系统发育方法适用于改进该属复杂的分类学。
