Xu Hai-Ying, Chen Li-Ping, Fu Song-Zhe, Fan Hong-Xia, Zhou Yu-Guang, Liu Shuang-Jiang, Liu Zhi-Pei
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2297-301. doi: 10.1099/ijs.0.004366-0. Epub 2009 Jul 20.
A Gram-negative, pale-yellow, non-spore-forming bacterium, motile with single polar flagella, strain E6T, was isolated from coastal seawater collected from Tianjin, China, and its taxonomic position was investigated using a polyphasic approach. Strain E6T requires NaCl for growth and grows optimally at pH 8.2 and 36 degrees C and in the presence of 2.0% (w/v) NaCl. It is positive for catalase and oxidase, and reduces nitrate to nitrite. The major fatty acids (>10%) are C18:1omega7c (64.71%) and 11-methyl C18:1omega7c (12.38%), the ubiquinone system is Q-10 and the DNA G+C content is 53.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain E6T represented a new lineage in the Alphaproteobacteria and is related to genera Cucumibacter, Devosia, Ochrobactrum and Ahrensia. Strain E6T shows highest 16S rRNA gene sequence similarity (93.3%) to Cucumibacter marinus CL-GR60T and less than 92.2% similarity to other relatives. It can be differentiated from its closest phylogenetic neighbours on the basis of several phenotypic features, including nitrate reduction, assimilation of D-glucose, L-arabinose, D-mannose, mannitol and maltose and major fatty acid composition. A polyphasic analysis supported the conclusion that strain E6T represents a novel genus and species of the family Hyphomicrobiaceae, for which the name Zhangella mobilis gen. nov., sp. nov. is proposed. The type strain of Zhangella mobilis is E6T (=CGMCC 1.7002T=JCM 15144T).
从中国天津沿海海水中分离出一株革兰氏阴性、淡黄色、不形成芽孢、具单极鞭毛可运动的细菌E6T，并采用多相分类法研究其分类地位。菌株E6T生长需要氯化钠，在pH 8.2、36℃以及2.0%（w/v）氯化钠存在的条件下生长最佳。过氧化氢酶和氧化酶呈阳性，可将硝酸盐还原为亚硝酸盐。主要脂肪酸（>10%）为C18:1ω7c（64.71%）和11-甲基C18:1ω7c（12.38%），泛醌系统为Q-10，DNA G+C含量为53.1 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株E6T代表了变形菌门α-变形菌纲中的一个新谱系，与黄瓜杆菌属、德沃斯氏菌属、苍白杆菌属和阿伦斯氏菌属相关。菌株E6T与海生黄瓜杆菌CL-GR60T的16S rRNA基因序列相似度最高（93.3%），与其他亲缘种的相似度低于92.2%。基于包括硝酸盐还原、D-葡萄糖、L-阿拉伯糖、D-甘露糖、甘露醇和麦芽糖的同化以及主要脂肪酸组成等几个表型特征，可将其与其最接近的系统发育邻种区分开来。多相分析支持菌株E6T代表了生丝微菌科一个新属和新种的结论，为此提出新属名张菌属（Zhangella）和新种名运动张菌（Zhangella mobilis）。运动张菌的模式菌株为E6T（=CGMCC 1.7002T=JCM 15144T）。
