Sheu Shih-Yi, Chiu Tzu Fang, Cho Nian-Tsz, Chou Jui-Hsing, Sheu Der-Shyan, Arun A B, Young Chiu-Chung, Chen Chaolun Allen, Wang Jih-Terng, Chen Wen-Ming
Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2546-51. doi: 10.1099/ijs.0.009738-0. Epub 2009 Jul 21.
Two Gram-negative, aerobic, pale pink/rose-coloured, rod-shaped, non-motile bacterial strains, designated GFA-11(T) and RG-4, were isolated from a freshwater fish culture pond and a freshwater spring sample, respectively. Based on characterization by using a polyphasic approach, the two strains showed highly similar phenotypic, physiological and genetic characteristics. They shared 99.9 % 16S rRNA gene sequence similarity and 89-94 % DNA-DNA relatedness, suggesting that they represent a single genomic species. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains GFA-11(T) and RG-4 formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Flectobacillus within the family Flexibacteraceae. Their closest neighbours were Flectobacillus major DSM 103(T) (97.7 % 16S rRNA gene sequence similarity) and Flectobacillus lacus CL-GP79(T) (95.9 %). Levels of DNA-DNA relatedness between the two novel strains and the type strains of F. major and F. lacus were less than 70 %. The results of physiological and biochemical tests allowed clear phenotypic differentiation of strains GFA-11(T) and RG-4 from recognized members of the genus Flectobacillus. The predominant fatty acid constituents of strain GFA-11(T) were C(16 : 1)omega5c (40.2 % of the total) and iso-C(15 : 0) (15.0 %). The DNA G+C content of strain GFA-11(T) was 39.7 mol%. On the basis of 16S rRNA gene sequence analysis and chemotaxonomic and physiological data, strains GFA-11(T) and RG-4 are considered to represent a novel species of the genus Flectobacillus, for which the name Flectobacillus roseus sp. nov. is proposed. The type strain is GFA-11(T) (=BCRC 17834(T)=LMG 24501(T)).
从一个淡水鱼养殖池塘和一个淡水泉样本中分别分离出两株革兰氏阴性、需氧、浅粉色/玫瑰色、杆状、无运动性的细菌菌株，分别命名为GFA-11(T)和RG-4。基于多相分类法的鉴定，这两株菌株表现出高度相似的表型、生理和遗传特征。它们的16S rRNA基因序列相似度为99.9%，DNA-DNA相关性为89 - 94%，表明它们代表一个单一的基因组物种。基于16S rRNA基因序列的系统发育分析表明，菌株GFA-11(T)和RG-4在弯曲杆菌科内弗氏杆菌属所占据的进化辐射边缘形成了一个单系分支。它们最接近的亲缘种是大弗氏杆菌DSM 103(T)（16S rRNA基因序列相似度为97.7%）和湖弗氏杆菌CL-GP79(T)（95.9%）。这两株新菌株与大弗氏杆菌和湖弗氏杆菌的模式菌株之间的DNA-DNA相关性水平低于70%。生理生化测试结果使得菌株GFA-11(T)和RG-4能够与弗氏杆菌属已确认的成员进行明确的表型区分。菌株GFA-11(T)的主要脂肪酸成分是C(16 : 1)ω5c（占总量的40.2%）和异-C(15 : 0)（15.0%）。菌株GFA-11(T)的DNA G+C含量为39.7 mol%。基于16S rRNA基因序列分析以及化学分类和生理数据，菌株GFA-11(T)和RG-4被认为代表弗氏杆菌属的一个新物种，为此提议命名为玫瑰弗氏杆菌（Flectobacillus roseus sp. nov.）。模式菌株是GFA-11(T)（=BCRC 17834(T)=LMG 24501(T)）。
