Sheu Shih-Yi, Chiu Tzu Fang, Chou Jui-Hsing, Sheu Der-Shyan, Arun A B, Young Chiu-Chung, Chen Chaolun Allen, Wang Jih-Terng, Chen Wen-Ming
Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2482-5. doi: 10.1099/ijs.0.009233-0. Epub 2009 Jul 21.
A bacterial strain designated GFC-1(T) was isolated from a fish-culture pond in Taiwan and was characterized by using the polyphasic taxonomic approach. Strain GFC-1(T) was Gram-negative, aerobic, rod-shaped, motile and non-spore-forming. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus Andreprevotia of the family Neisseriaceae and its closest neighbour was Andreprevotia chitinilytica JS11-7(T) (97.0 % sequence similarity). The results of physiological and biochemical tests allowed clear phenotypic differentiation of isolate GFC-1(T) from A. chitinilytica JS11-7(T). The major fatty acids were summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH), C(16 : 0) and C(18 : 1)omega7c. The DNA G+C content was 63.0 mol%. On the basis of 16S rRNA gene sequence analysis and chemotaxonomic and physiological data, strain GFC-1(T) should be classified as representing a novel species and a second member of the genus Andreprevotia, for which the name Andreprevotia lacus sp. nov. is proposed. The type strain is GFC-1(T) (=BCRC 17832(T)=LMG 24502(T)).
从台湾一个养鱼池塘中分离出一株命名为GFC-1(T)的细菌菌株，并采用多相分类法对其进行了特征描述。菌株GFC-1(T)为革兰氏阴性、需氧、杆状、具运动性且不形成芽孢。基于16S rRNA基因序列的系统发育分析表明，该菌株属于奈瑟菌科的Andreprevotia属，其最接近的亲缘种是解几丁质Andreprevotia chitinilytica JS11-7(T)（序列相似性为97.0%）。生理生化测试结果使分离株GFC-1(T)与解几丁质Andreprevotia chitinilytica JS11-7(T)能够在表型上明确区分。主要脂肪酸为总和特征3（C(16 : 1)ω7c和/或异-C(15 : 0) 2-OH）、C(16 : 0)和C(18 : 1)ω7c。DNA G+C含量为63.0 mol%。基于16S rRNA基因序列分析、化学分类学和生理学数据，菌株GFC-1(T)应被分类为代表一个新物种，也是Andreprevotia属的第二个成员，为此提议将其命名为湖生Andreprevotia lacus sp. nov.。模式菌株为GFC-1(T)（=BCRC 17832(T)=LMG 24502(T)）。
