Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
Hippocampus. 2010 Jul;20(7):811-9. doi: 10.1002/hipo.20682.
This study aimed at an analysis of expression of epidermal-type and brain-type fatty acid-binding proteins (E-FABP and B-FABP, also called FABP5 and FABP7, respectively) in adult hippocampus and their potential value as neuroprotective factors after ischemic brain damage in monkey model. The immunostaining and Western blotting results show that FABP5 was mainly expressed in neurons, whereas FABP7 was primarily expressed in astrocytes and progenitors of the subgranular zone (SGZ). Interestingly, FABP5 expression in neurons increased in cornu Ammonis 1 (CA1) and remains stable within dentate gyrus (DG) after ischemia; FABP7 expression increased within both CA1 and SGZ. This indicates a potential role for FABP5 and FABP7 in intracellular fatty acid transport within different neural cells. The change in FABP5-7 expression within CA1 and DG of the adult postischemic hippocampus was compatible with previous findings of downregulation in CA1 neurons and upregulation in SGZ progenitor cells after ischemia. Altogether, the present data suggest that polyunsaturated fatty acids, such as docosahexaenoic acid, may act via FABP5 or 7 to regulate adult postischemic hippocampal neuronal antiapoptosis or neurogenesis in primates.
本研究旨在分析表皮型和脑型脂肪酸结合蛋白（E-FABP 和 B-FABP，也分别称为 FABP5 和 FABP7）在成年海马体中的表达及其作为猴模型缺血性脑损伤后神经保护因子的潜在价值。免疫染色和 Western blot 结果表明，FABP5 主要在神经元中表达，而 FABP7 主要在星形胶质细胞和颗粒下区（SGZ）的祖细胞中表达。有趣的是，神经元中 FABP5 的表达在缺血后 CA1 区增加，而在齿状回（DG）中保持稳定；FABP7 的表达在 CA1 和 SGZ 中均增加。这表明 FABP5 和 FABP7 在不同神经细胞内的细胞内脂肪酸转运中具有潜在作用。FABP5-7 在成年缺血后海马体 CA1 和 DG 中的表达变化与以前的研究结果一致，即 CA1 神经元下调和 SGZ 祖细胞上调。总的来说，本研究数据表明，多不饱和脂肪酸，如二十二碳六烯酸，可能通过 FABP5 或 FABP7 来调节灵长类动物缺血后成年海马体神经元的抗细胞凋亡或神经发生。
