Department of Restorative Neurosurgery, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Neurosci Res. 2010 Oct;68(2):94-102. doi: 10.1016/j.neures.2010.07.2028. Epub 2010 Jul 8.
To clarify the involvement of fatty acid binding proteins (FABPs) in cerebellar development and function, we explored the distribution of three brain-expressed FABPs, FABP 3, 5 and 7, by comparing three animal groups--infantile, normal and postischemic adult monkeys. Immunoblotting analysis revealed intense expression of FABP 3 and 7, but not of FABP5, in the control and postischemic adult cerebellum. The protein levels of FABP7, but not of FABP 3 or 5, gradually increased until 2 weeks after the insult. Immunohistochemical analysis showed that cerebellar FABP3-positive cells were Purkinje cells and Bergmann glia. FABP5-positive cells were found only in the postischemic cerebellum, and were identified as activated microglia. Interestingly, in the infantile cerebellum, both the granule cell progenitors in the external granular layer (EGL) and the oligodendrocyte progenitors in the internal granular layer (IGL) expressed FABP5. In the adult cerebellum, FABP7 was expressed in Purkinje cells and basket interneurons, while in the infantile cerebellum it was also found in Bergmann glia. These results showed differential expression of FABPs in cerebellar neuronal and glial cell types; FABP 3 and 7 were predominantly expressed in normal cerebellum, FABP5 after ischemic injury, while FABP 3, 5 and 7 were expressed during cerebellar development.
为了阐明脂肪酸结合蛋白（FABP）在小脑发育和功能中的作用，我们通过比较三组动物（婴儿期、正常和缺血后成年期猴子），研究了三种脑表达的 FABP（FABP3、5 和 7）的分布。免疫印迹分析显示，FABP3 和 7 在对照和缺血后成年小脑中有强烈表达，但 FABP5 没有表达。FABP7 的蛋白水平逐渐增加，直到损伤后 2 周。免疫组织化学分析显示，小脑 FABP3 阳性细胞为浦肯野细胞和伯格曼胶质细胞。FABP5 阳性细胞仅在缺血后小脑中发现，被鉴定为激活的小胶质细胞。有趣的是，在婴儿小脑中，外颗粒层（EGL）中的颗粒细胞前体细胞和内颗粒层（IGL）中的少突胶质细胞前体细胞都表达 FABP5。在成年小脑中，FABP7 表达在浦肯野细胞和篮状中间神经元中，而在婴儿小脑中也发现表达在伯格曼胶质细胞中。这些结果显示 FABP 在小脑神经元和神经胶质细胞类型中的表达存在差异；FABP3 和 7 在正常小脑中主要表达，FABP5 在缺血损伤后表达，而 FABP3、5 和 7 在小脑发育过程中表达。
