Dzikwi A, Umoh J U, Kwaga J K P, Ahmad A A, deBeer M, Steele A D
Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, Zaria, Nigeria.
Ann Afr Med. 2008 Dec;7(4):163-7. doi: 10.4103/1596-3519.55659.
It is estimated that about 600,000 children die annually as a result of severe dehydrating diarrhea caused by rotaviruses. The virus is a double stranded RNA (dsRNA) virus with 11 segments. Group A rotaviruses show a characteristic 4-2-3-2 pattern following electrophoresis. The VP6 subgroups, I and II exist. This work was carried out to study the prevalence of rotavirus infection among children 0-5 years with diarrhea in Kano, and to determine the circulating subgroups and electropherotypes and of the rotavirus isolates.
Two hundred and eighteen stool specimens from children 0-60 months (198 diarrheic and 20 non-diarrheic) were collected from different hospitals and health care centers in Kano and subjected to group A rotavirus enzyme linked immunosorbent assay (ELISA) to determine presence of group A rotavirus, subgroup ELISA to determine the VP6 subgroups and polyacrylamide gel electrophoresis (PAGE) to determine the electropherotypes present.
The long electropherotypes (47.05%) of four variations dominated over the short electropherotype (17.64%). About 11.76% of the isolates were of mixed infection. Dominance of subgroup II (45%) over subgroup I (25%), and the presence of both subgroups I and II (10%) and neither subgroup I nor II (15%) was observed in this study.
Information on the genomic diversity of the RNA electropherotypes in this region, Kano, is reported in this study.
据估计，每年约有60万儿童死于轮状病毒引起的严重脱水腹泻。该病毒是一种具有11个节段的双链RNA（dsRNA）病毒。A组轮状病毒在电泳后呈现出特征性的4-2-3-2模式。存在VP6亚组I和II。开展这项工作是为了研究卡诺0至5岁腹泻儿童中轮状病毒感染的患病率，并确定轮状病毒分离株的流行亚组、电泳图谱类型。
从卡诺的不同医院和医疗保健中心收集了218份0至60个月儿童的粪便标本（198份腹泻标本和20份非腹泻标本），并进行A组轮状病毒酶联免疫吸附测定（ELISA）以确定A组轮状病毒的存在，进行亚组ELISA以确定VP6亚组，并进行聚丙烯酰胺凝胶电泳（PAGE）以确定存在的电泳图谱类型。
四种变异的长电泳图谱类型（47.05%）占主导地位，短电泳图谱类型（17.64%）较少。约11.76%的分离株为混合感染。在本研究中观察到II亚组（45%）比I亚组（25%）占优势，同时存在I亚组和II亚组（10%）以及既不存在I亚组也不存在II亚组（15%）的情况。
本研究报告了卡诺这一地区RNA电泳图谱类型的基因组多样性信息。
