el Assouli S M, Mohammed K A, Banjar Z M
Department of Medical Biology, King Abdulaziz University, Jeddah, Saudi Arabia.
Ann Trop Paediatr. 1995;15(1):45-53. doi: 10.1080/02724936.1995.11747748.
Polyacrylamide gel electrophoresis was used to analyze the double-stranded RNA of rotavirus recovered from stool specimens collected between March 1988 and December 1992 from children with acute diarrhoea admitted to the central hospital in Jeddah, Saudi Arabia. Rotavirus was detected by ELISA in 523 specimens of the 1242 collected during the study, giving an overall prevalence of 42.2% for the 5 years of investigation. Out of 523 rotavirus-positive specimens, 263 (50.3%) were successfully electropherotyped. Eighteen different electropherotypes were observed, 12 long patterns and six short patterns. Eighty-seven per cent of the electropherotypes were of the long RNA pattern. The remaining were short electropherotypes. The predominant electropherotypes were two long patterns and maintained dominance over 5 years of the study. The third most predominant pattern was a short electropherotype. The remaining electropherotypes made minor contributions every year. These predominant electropherotypes were different from the predominant patterns detected in many countries. Temporal distribution was observed for 5 successive years for the predominant electropherotypes, with an increase in the incidence during the cooler months of the year.
采用聚丙烯酰胺凝胶电泳分析1988年3月至1992年12月期间从沙特阿拉伯吉达中央医院收治的急性腹泻儿童粪便标本中回收的轮状病毒双链RNA。在研究期间收集的1242份标本中，通过ELISA检测出523份标本含有轮状病毒，5年调查的总体患病率为42.2%。在523份轮状病毒阳性标本中，263份（50.3%）成功进行了电泳分型。观察到18种不同的电泳型，12种长型和6种短型。87%的电泳型为长RNA型。其余为短电泳型。主要的电泳型为两种长型，在5年的研究中保持优势。第三大主要类型是短电泳型。其余的电泳型每年的贡献较小。这些主要的电泳型与许多国家检测到的主要类型不同。对主要电泳型连续5年观察到时间分布，在一年中较凉爽的月份发病率增加。
