Maldonado A J, Bastardo J W
Postgrado en Biología Aplicada, Universidad de Oriente, Núcleo de Sucre, Cumaná, Estado Sucre, Venezuela.
Invest Clin. 1998 Sep;39(3):39-51.
From June 1992 to May 1993, rotaviruses were detected by an immunoenzymatic assay in 159 (49.5%) of 321 children admitted to the hospital with acute diarrhea. Of the 159 cases ELISA positive, 80 samples were chosen at random to investigate subgroups and serotypes of group A human rotavirus. By the ELISA test 9 (11.3%) of the strains were subgroup I, 46 (57.5%) were subgroup II, and 25 (31.3%) could not be grouped. The serotype G1 was identified in 52 cases (65%), G2 in 11 cases (13.8%), G3 in 1 case (1.2%), and 7 cases (8.8%) showed more than one serotype. By electrophoretic analysis of viral RNA, 137 (42.7%) of the samples exhibited an RNA pattern. The long pattern (59.1%) prevailed over the short pattern (35.8%), and by coelectrophoresis 8 different electropherotypes were found throughout the period of study. These results illustrate the great variety of rotavirus strains in this region of the country.
1992年6月至1993年5月，采用免疫酶法对321例因急性腹泻入院的儿童进行检测，发现159例（49.5%）感染轮状病毒。在159例ELISA检测呈阳性的病例中，随机选取80份样本，对A组人轮状病毒的亚组和血清型进行调查。通过ELISA检测，9株（11.3%）为I亚组，46株（57.5%）为II亚组，25株（31.3%）无法分型。52例（65%）鉴定为G1血清型，11例（13.8%）为G2血清型，1例（1.2%）为G3血清型，7例（8.8%）呈现多种血清型。通过对病毒RNA进行电泳分析，137份样本（42.7%）呈现RNA图谱。长型图谱（59.1%）多于短型图谱（35.8%），在整个研究期间通过共电泳发现8种不同的电泳型。这些结果表明该国该地区轮状病毒株具有多样性。
