不同树龄及不同采收季节银杏叶中总银杏酸动态变化的研究
[Studies on dynamic change of total ginkgolic acids in Ginkgo biloba leaves of different aged trees and different collecting seasons].
作者信息
Ju Jianming, Huang Yiping, Qian Shihui, Qian Dawei, Duan Jin'ao, Cao Fuliang
机构信息
Jiangsu Academy of Traditional Chinese Medicine, Nanjing 210028, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Apr;34(7):817-9.
OBJECTIVE
To study dynamic change of total ginkgolic acids in Ginkgo biloba leaves of the different aged trees and different collecting seasons.
METHOD
The content of total ginkgolic acids in G. biloba leaves was determined by HPLC. A Alltima C18 (4.6 mm x 250 mm, 5 microm) and the mobile phase of methanol and 1% acetic acid (90:10) were used, the flow rate was 1.0 mL x min(-1), and the wavelength was 310 nm. The content were calculated with external standard method.
RESULT
The content of total ginkgolic acids in G. biloba leaves was in the range of 0.48% to 2.51% in different collecting seasons. The content reached maximum at the end of May and the beginning of June, and then declined gradually. In different aged trees, the content in the older ages was lower than that in the younger ages.
CONCLUSION
The results provide scientific basis for the collecting season of G. biloba leaves.
目的
研究不同树龄和不同采收季节银杏叶中总银杏酸的动态变化。
方法
采用高效液相色谱法测定银杏叶中总银杏酸的含量。使用Alltima C18（4.6 mm×250 mm，5微米）色谱柱，以甲醇和1%乙酸（90:10）为流动相，流速为1.0 mL·min⁻¹，检测波长为310 nm。采用外标法计算含量。
结果
不同采收季节银杏叶中总银杏酸的含量在0.48%至2.51%之间。5月底至6月初含量达到最高，随后逐渐下降。在不同树龄的银杏叶中，树龄较大的银杏叶中总银杏酸含量低于树龄较小的。
结论
该研究结果为银杏叶的采收季节提供了科学依据。
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