超高效液相色谱-四极杆飞行时间质谱法分析贵州杜鹃中的黄酮类化合物
[Analysis of flavonoids in Rhododendron mariae by UPLC/ Q-TOF-MS].
作者信息
Huang Huiqiang, Feng Yifan, Rui Wen, Jiang Miaomiao, Han Liang
机构信息
Central Laboratory of Guangdong Pharmaceutical University, Guangzhou 510006, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Apr;34(7):875-8.
OBJECTIVE
To investigate the flavonoids in Rhododendron mariae.
METHOD
The constituents in R. mariae were determined by UPLC/Q-TOF-MS. The chromatographic separation was performed on a C18 column (2.1 mm x 50 mm, 1.7 microm) with a gradient elufion of methanol-water containing 0.1% formic acid. The mass specfrometer eqaipped with elecfrospay ionizafion source was usedas defecfor and operated in data was collected under the positive and negative ion modes.
RESULT
Seven constituents were identified as myricetin-3-O-beta-D-gluconside (1), myricetin-3'-O-beta-D-xylopyraoside (2), hyperoside (3), isoquercitrin (4), avicularin (5), quercitroside (6) and quercetin (7).
CONCLUSION
In this study, the main flavonoids in R. mariae were separated by UPLC, and identified through the information of positive ion and negative ion and relative molecular mass which were determined by Q-TOF-MS. It is an accurate and effective method which can be applied for the constituent identification of R. mariae.
目的
研究岭南杜鹃中的黄酮类化合物。
方法
采用超高效液相色谱/四极杆飞行时间质谱（UPLC/Q-TOF-MS）测定岭南杜鹃中的成分。色谱分离在C18柱（2.1 mm×50 mm，1.7 µm）上进行，以含0.1%甲酸的甲醇-水梯度洗脱。采用配备电喷雾电离源的质谱仪作为检测器，在正离子和负离子模式下采集数据。
结果
鉴定出7种成分，分别为杨梅素-3-O-β-D-葡萄糖苷（1）、杨梅素-3'-O-β-D-木糖苷（2）、金丝桃苷（3）、异槲皮苷（4）、扁蓄苷（5）、槲皮苷（6）和槲皮素（7）。
结论
本研究采用UPLC分离岭南杜鹃中的主要黄酮类化合物，并通过Q-TOF-MS测定的正离子、负离子信息及相对分子质量进行鉴定。该方法准确有效，可用于岭南杜鹃成分的鉴定。
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