Shan Mingqiu, Qian Wen, Gao Jing, Chi Yumei, Zhang Li, Ding Anwei
Nanjing University of Chinese Medicine, Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing 210046, China.
Zhongguo Zhong Yao Za Zhi. 2011 Jun;36(12):1626-9.
To investigate the flavonoids in Platycladi Cacumen.
The constituents in Platycladi Cacumen were determined by UPLC-MS. A Waters BEH C18 column (2.1 mm x 150 mm, 1.7 microm) was used with a gradient elution of methanol-water containing 0.2% formic acid. The mass spectrometer equipped with electrospay ionization source was used as defector and operated in data was collected under the negative ion modes.
Eleven constituents were identified.
In this study, the main flavonoids in Platycladi Cacumen were separated by UPLC, and identified through the information of mass number and UV spectra. With the information of MS/MS from flavonoids, the modes of breaking up flavonoids were discussed. It is an accurate and effective method which can be applied for the constituent identification of Platycladi Cacumen.
研究侧柏叶中的黄酮类化合物。
采用超高效液相色谱-质谱联用（UPLC-MS）法测定侧柏叶中的成分。使用沃特世BEH C18色谱柱（2.1 mm×150 mm，1.7μm），以含0.2%甲酸的甲醇-水进行梯度洗脱。配备电喷雾电离源的质谱仪作为检测器，在负离子模式下采集数据。
鉴定出11种成分。
本研究通过UPLC分离了侧柏叶中的主要黄酮类化合物，并根据质谱数和紫外光谱信息进行了鉴定。结合黄酮类化合物的二级质谱信息，探讨了黄酮类化合物的裂解方式。这是一种准确有效的方法，可用于侧柏叶成分的鉴定。
