Redding W R, Booth L C
Department of Veterinary Clinical Sciences, Veterinary Teaching Hospital, Iowa State University, Ames.
Vet Surg. 1991 Sep-Oct;20(5):306-10. doi: 10.1111/j.1532-950x.1991.tb01272.x.
Equine fibroblasts and Staphylococcus aureus were exposed for 30 minutes to six dilutions of chlorhexidine gluconate, a chlorous acid-chlorine dioxide irrigation solution, a chlorous acid-chlorine dioxide disinfectant, and phosphate buffered saline controls. Cell viability was determined by trypsinizing the cells, staining them with trypan blue, and counting cells that did not take the stain. All fibroblasts were killed when exposed to 1.0% and 0.5% chlorhexidine. The survival rate of fibroblasts increased linearly with decreasing concentrations of chlorhexidine gluconate, with a peak survival of 50% at 0.005% chlorhexidine. The chlorous acid-chlorine dioxide irrigation solution was the least toxic to fibroblasts, with survival rates equivalent to those of controls. The chlorous acid-chlorine dioxide disinfectant was 100% cytotoxic even when diluted 1:1 with phosphate buffered saline. S. aureus growth was inhibited by 1.0% and 0.5% chlorhexidine gluconate; concentrations of 0.05%, 0.01%, and 0.005% did not differ from sterile water controls. The chlorous acid-chlorine dioxide irrigation solution did not inhibit growth of S. aureus in brain-heart infusion broth. The chlorous acid-chlorine dioxide disinfectant inhibited growth of S. aureus.
将马成纤维细胞和金黄色葡萄球菌暴露于葡萄糖酸氯己定的六种稀释液、一种亚氯酸 - 二氧化氯冲洗液、一种亚氯酸 - 二氧化氯消毒剂以及磷酸盐缓冲盐水对照中30分钟。通过用胰蛋白酶消化细胞、用台盼蓝染色并计数未被染色的细胞来测定细胞活力。当暴露于1.0%和0.5%的葡萄糖酸氯己定时，所有成纤维细胞均被杀死。成纤维细胞的存活率随着葡萄糖酸氯己定浓度的降低呈线性增加，在0.005%葡萄糖酸氯己定时存活率峰值为50%。亚氯酸 - 二氧化氯冲洗液对成纤维细胞的毒性最小，存活率与对照组相当。即使与磷酸盐缓冲盐水按1:1稀释，亚氯酸 - 二氧化氯消毒剂仍具有100%的细胞毒性。1.0%和0.5%的葡萄糖酸氯己定可抑制金黄色葡萄球菌的生长；0.05%、0.01%和0.005%的浓度与无菌水对照无差异。亚氯酸 - 二氧化氯冲洗液在脑心浸液肉汤中不抑制金黄色葡萄球菌的生长。亚氯酸 - 二氧化氯消毒剂可抑制金黄色葡萄球菌的生长。
