Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Department of Oral Biology, Semmelweis University, Budapest, Hungary.
Clin Oral Investig. 2021 May;25(5):2981-2992. doi: 10.1007/s00784-020-03618-5. Epub 2020 Oct 12.
Periodontal ligament stem cells (PDLSCs) have an underlined significance as their high proliferative capacity and multipotent differentiation provide an important therapeutic potential. The integrity of these cells is frequently disturbed by the routinely used irrigative compounds applied as periodontal or endodontic disinfectants (e.g., hydrogen peroxide (HO) and chlorhexidine (CHX)). Our objectives were (i) to monitor the cytotoxic effect of a novel dental irrigative compound, chlorine dioxide (ClO), compared to two traditional agents (HO, CHX) on PDLSCs and (ii) to test whether the aging factor of PDLSC cultures determines cellular responsiveness to the chemicals tested.
Impedimetry (concentration-response study), WST-1 assays (WST = water soluble tetrazolium salt), and morphology analysis were performed to measure changes in cell viability induced by the 3 disinfectants; immunocytochemistry of stem cell markers (STRO-1, CD90, and CD105) measured the induced mesenchymal characteristics.
Cell viability experiments demonstrated that the application of ClO does not lead to a significant decrease in viability of PLDSCs in concentrations used to kill microbes. On the contrary, traditional irrigants, HO, and CHX are highly toxic on PDLSCs. Aging of PLDSC cultures (passages 3 vs. 7) has characteristic effects on their responsiveness to these agents as the increased expression of mesenchymal stem cell markers turns to decreased.
While the active ingredients of mouthwash (HO, CHX) applied in endodontic or periodontitis management have a serious toxic effect on PDLSCs, the novel hyperpure ClO is less toxic providing an environment favoring dental structure regenerations during disinfectant interventions.
牙周韧带干细胞（PDLSCs）具有重要的治疗潜力，其高增殖能力和多能分化特性备受关注。这些细胞的完整性经常受到作为牙周或牙髓消毒剂常规使用的冲洗化合物的干扰（例如，过氧化氢（HO）和洗必泰（CHX））。我们的目的是（i）监测新型牙科冲洗化合物二氧化氯（ClO）对 PDLSCs 的细胞毒性作用，并与两种传统试剂（HO、CHX）进行比较，以及（ii）测试 PDLSC 培养物的老化因素是否决定细胞对测试化学品的反应性。
使用阻抗测定法（浓度反应研究）、WST-1 测定法（WST =水溶性四唑盐）和形态分析来测量 3 种消毒剂引起的细胞活力变化；通过干细胞标志物（STRO-1、CD90 和 CD105）的免疫细胞化学来测量诱导的间充质特征。
细胞活力实验表明，ClO 的应用不会导致在用于杀死微生物的浓度下 PLDSC 活力显著降低。相反，传统的冲洗剂 HO 和 CHX 对 PDLSCs 具有高度毒性。PLDSC 培养物的老化（第 3 代与第 7 代）对它们对这些试剂的反应性具有特征性影响，因为间充质干细胞标志物的表达增加转化为降低。
虽然应用于牙髓病或牙周炎治疗的漱口剂（HO、CHX）的有效成分对 PDLSCs 具有严重的毒性作用，但新型高纯 ClO 的毒性较小，为在消毒剂干预期间促进牙齿结构再生提供了有利的环境。
