在狼疮小鼠模型中，I型干扰素受体控制B细胞中核酸感应Toll样受体的表达及自身抗体的产生。
Type I interferon receptor controls B-cell expression of nucleic acid-sensing Toll-like receptors and autoantibody production in a murine model of lupus.
作者信息
Thibault Donna L, Graham Kareem L, Lee Lowen Y, Balboni Imelda, Hertzog Paul J, Utz Paul J
机构信息
Department of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA.
出版信息
Arthritis Res Ther. 2009;11(4):R112. doi: 10.1186/ar2771. Epub 2009 Jul 22.
INTRODUCTION
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by the production of high-titer IgG autoantibodies directed against nuclear autoantigens. Type I interferon (IFN-I) has been shown to play a pathogenic role in this disease. In the current study, we characterized the role of the IFNAR2 chain of the type I IFN (IFN-I) receptor in the targeting of nucleic acid-associated autoantigens and in B-cell expression of the nucleic acid-sensing Toll-like receptors (TLRs), TLR7 and TLR9, in the pristane model of lupus.
METHODS
Wild-type (WT) and IFNAR2-/- mice were treated with pristane and monitored for proteinuria on a monthly basis. Autoantibody production was determined by autoantigen microarrays and confirmed using enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation. Serum immunoglobulin isotype levels, as well as B-cell cytokine production in vitro, were quantified by ELISA. B-cell proliferation was measured by thymidine incorporation assay.
RESULTS
Autoantigen microarray profiling revealed that pristane-treated IFNAR2-/- mice lacked autoantibodies directed against components of the RNA-associated autoantigen complexes Smith antigen/ribonucleoprotein (Sm/RNP) and ribosomal phosphoprotein P0 (RiboP). The level of IgG anti-single-stranded DNA and anti-histone autoantibodies in pristane-treated IFNAR2-/- mice was decreased compared to pristane-treated WT mice. TLR7 expression and activation by a TLR7 agonist were dramatically reduced in B cells from IFNAR2-/- mice. IFNAR2-/- B cells failed to upregulate TLR7 as well as TLR9 expression in response to IFN-I, and effector responses to TLR7 and TLR9 agonists were significantly decreased as compared to B cells from WT mice following treatment with IFN-alpha.
CONCLUSIONS
Our studies provide a critical link between the IFN-I pathway and the regulation of TLR-specific B-cell responses in a murine model of SLE.
引言
系统性红斑狼疮（SLE）是一种慢性自身免疫性疾病，其特征是产生针对核自身抗原的高滴度IgG自身抗体。I型干扰素（IFN-I）已被证明在该疾病中起致病作用。在本研究中，我们在狼疮的 pristane 模型中，表征了 I 型干扰素（IFN-I）受体的 IFNAR2 链在核酸相关自身抗原靶向以及核酸感应 Toll 样受体（TLR）TLR7 和 TLR9 的 B 细胞表达中的作用。
方法
用 pristane 处理野生型（WT）和 IFNAR2 - / - 小鼠，并每月监测蛋白尿情况。通过自身抗原微阵列确定自身抗体产生，并使用酶联免疫吸附测定（ELISA）和免疫沉淀进行确认。通过 ELISA 定量血清免疫球蛋白同种型水平以及体外 B 细胞细胞因子产生。通过胸苷掺入试验测量 B 细胞增殖。
结果
自身抗原微阵列分析显示，用 pristane 处理的 IFNAR2 - / - 小鼠缺乏针对与 RNA 相关的自身抗原复合物史密斯抗原/核糖核蛋白（Sm/RNP）和核糖体磷蛋白 P0（RiboP）成分的自身抗体。与用 pristane 处理的 WT 小鼠相比，用 pristane 处理的 IFNAR2 - / - 小鼠中 IgG 抗单链 DNA 和抗组蛋白自身抗体水平降低。IFNAR2 - / - 小鼠的 B 细胞中 TLR7 激动剂诱导的 TLR7 表达和激活显著降低。IFNAR2 - / - B 细胞在响应 IFN-I 时未能上调 TLR7 以及 TLR9 的表达，并且与用 IFN-α 处理后的 WT 小鼠的 B 细胞相比，对 TLR7 和 TLR9 激动剂的效应反应显著降低。
结论
我们的研究在 SLE 小鼠模型中提供了 IFN-I 途径与 TLR 特异性 B 细胞反应调节之间的关键联系。
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