Yang Yan, Lu Shi, Li Min-Fang, Wang Ze-Hua
Department of Obstetrics and Gynecology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
Ai Zheng. 2009 Jul;28(7):702-7. doi: 10.5732/cjc.008.10719.
Previous studies have shown that nm23-H1 is a tumor metastasis suppressor gene. Nucleotide diphosphate kinase 1 (NDPK1) encoded by nm23-H1 is involved in cancer cellular differentiation, proliferation, apoptosis and metastasis. This study was to investigate the effects of nm23-H1 on proliferation and invasion of cervical cancer cells.
The eukaryotic expression vector pcDNA3.1-nm23-H1 was transfected into cervical cancer cells. Cell invasion potential was determined by the Transwell assay. Cell proliferation was measured by MTT assay and changes in cell cycle distribution were analyzed by flow cytometry (FCM).
Compared with parent cells (Caski and SiHa) and vector control cells (Caski-3.1 and SiHa-3.1), the proliferation and invasion of pcDNA3.1-nm23-H1 transfected cells (SiHa-N and Caski-N) were apparently decreased (P<0.05); the proportions of G2/M and S cells were obviously decreased while that of G0/G1 cells was increased (P<0.05). However, transfection of nm23-H1 gene had no influence on proliferation, cell cycle and invasion of HeLa cells (P>0.05).
nm23-H1 gene could inhibit proliferation and invasion of cervical cancer cells in a cell-dependent manner.
既往研究表明nm23-H1是一种肿瘤转移抑制基因。由nm23-H1编码的核苷二磷酸激酶1（NDPK1）参与癌细胞的分化、增殖、凋亡及转移。本研究旨在探讨nm23-H1对宫颈癌细胞增殖和侵袭的影响。
将真核表达载体pcDNA3.1-nm23-H1转染至宫颈癌细胞。采用Transwell实验测定细胞侵袭能力。通过MTT实验检测细胞增殖情况，并采用流式细胞术（FCM）分析细胞周期分布的变化。
与亲本细胞（Caski和SiHa）及载体对照细胞（Caski-3.1和SiHa-3.1）相比，pcDNA3.1-nm23-H1转染细胞（SiHa-N和Caski-N）的增殖和侵袭能力明显降低（P<0.05）；G2/M期和S期细胞比例明显降低，而G0/G1期细胞比例增加（P<0.05）。然而，nm23-H1基因转染对HeLa细胞的增殖、细胞周期及侵袭无影响（P>0.05）。
nm23-H1基因可依细胞类型抑制宫颈癌细胞的增殖和侵袭。
