Niu Lili, Song Lei, Liu Xiaoli, Dong Xiuzhu
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2698-701. doi: 10.1099/ijs.0.005124-0. Epub 2009 Jul 22.
A novel, xylanolytic, anaerobic, moderately thermophilic bacterium, strain PML14(T), was isolated from the sludge of a thermophilic anaerobic digester treating municipal solid waste and sewage in Beijing, China. The strain was a Gram-positive, spore-forming and motile rod. Growth of the novel strain was observed at 25-67 degrees C (optimum 60 degrees C) and pH 5.8-9.3 (optimum pH 8.5). Strain PML14(T) grew on a number of carbohydrates, including xylan, xylose, glucose and cellobiose, and a variety of proteinaceous compounds, including peptone, tryptone, Casamino acids, yeast extract, beef extract, casein hydrolysate, l-cysteine, l-serine, l-lysine, l-glycine, l-threonine, l-methionine and pyruvate. The fermentation products from glucose included acetate, ethanol, butyrate, hydrogen and carbon dioxide. Propionate was produced from xylan in addition to other compounds. Fe(III), 9,10-anthraquinone 2,6-disulfonate and thiosulfate were reduced with peptone as the electron donor. NH(3) was produced. Indole was not produced. Gelatin was not hydrolysed. The DNA G+C content of strain PML14(T) was 36.2+/-0.8 mol% (T(m)). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PML14(T) was related to the members of cluster XII of the clostridia, most closely to Tepidimicrobium ferriphilum SB91(T) with 93.8 % 16S rRNA gene sequence similarity. On the basis of polyphasic evidence from this study, it is suggested that strain PML14(T) (=CGMCC 1.5080(T)=JCM 15035(T)) represents a novel species of the genus Tepidimicrobium, for which the name Tepidimicrobium xylanilyticum sp. nov., is proposed. An emended description of the genus Tepidimicrobium is also provided.
从中国北京处理城市固体废物和污水的嗜热厌氧消化池污泥中分离出一株新型的、具有木聚糖分解能力的厌氧嗜热细菌，菌株PML14(T)。该菌株为革兰氏阳性、产芽孢且具运动性的杆菌。在25 - 67℃（最适温度60℃）和pH 5.8 - 9.3（最适pH 8.5）条件下可观察到该新型菌株生长。菌株PML14(T)能利用多种碳水化合物生长，包括木聚糖、木糖、葡萄糖和纤维二糖，以及多种含氮化合物，包括蛋白胨、胰蛋白胨、酪蛋白氨基酸、酵母提取物、牛肉提取物、酪蛋白水解物、L - 半胱氨酸、L - 丝氨酸、L - 赖氨酸、L - 甘氨酸、L - 苏氨酸、L - 甲硫氨酸和丙酮酸。葡萄糖发酵产物包括乙酸盐、乙醇、丁酸盐、氢气和二氧化碳。除其他化合物外，木聚糖还可产生丙酸盐。以蛋白胨作为电子供体时，Fe(III)、9,10 - 蒽醌 - 2,6 - 二磺酸盐和硫代硫酸盐可被还原。可产生NH₃。不产生吲哚。不水解明胶。菌株PML14(T)的DNA G + C含量为36.2±0.8 mol%（Tm）。基于16S rRNA基因序列的系统发育分析表明，菌株PML14(T)与梭菌属XII簇成员相关，与嗜铁温微菌SB91(T)关系最为密切，16S rRNA基因序列相似性为93.8%。基于本研究的多相证据，建议菌株PML14(T)（=CGMCC 1.5080(T)=JCM 15035(T)）代表温微菌属的一个新物种，为此提出新名称嗜木温微菌（Tepidimicrobium xylanilyticum sp. nov.）。还提供了温微菌属的修订描述。
