在模拟微重力条件下无白血病抑制因子的胚胎干细胞培养
LIF-free embryonic stem cell culture in simulated microgravity.
作者信息
Kawahara Yumi, Manabe Tomotaka, Matsumoto Masaya, Kajiume Teruyuki, Matsumoto Masayasu, Yuge Louis
机构信息
Department of Clinical Neuroscience and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan.
出版信息
PLoS One. 2009 Jul 23;4(7):e6343. doi: 10.1371/journal.pone.0006343.
BACKGROUND
Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required.
METHODOLOGY/PRINCIPAL FINDINGS: We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF.
CONCLUSIONS/SIGNIFICANCE: Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells.
背景
白血病抑制因子（LIF）是维持小鼠胚胎干细胞（ES细胞）多能性必不可少的因子。维持未分化状态还需要饲养层和血清，然而，临床应用需要去除这些动物源性材料。因此，需要一种更可靠的ES细胞培养技术。
方法/主要发现：我们使用三维回转器在模拟微重力条件下培养小鼠ES细胞。我们使用了不含LIF的无饲养层和无血清培养基。
结论/意义：在此我们表明，模拟微重力允许采用新型的无LIF和无动物源性材料的方法来培养小鼠ES细胞。
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