Kawahara Yumi, Manabe Tomotaka, Matsumoto Masaya, Kajiume Teruyuki, Matsumoto Masayasu, Yuge Louis
Department of Clinical Neuroscience and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan.
PLoS One. 2009 Jul 23;4(7):e6343. doi: 10.1371/journal.pone.0006343.
Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required.
METHODOLOGY/PRINCIPAL FINDINGS: We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF.
CONCLUSIONS/SIGNIFICANCE: Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells.
白血病抑制因子（LIF）是维持小鼠胚胎干细胞（ES细胞）多能性必不可少的因子。维持未分化状态还需要饲养层和血清，然而，临床应用需要去除这些动物源性材料。因此，需要一种更可靠的ES细胞培养技术。
方法/主要发现：我们使用三维回转器在模拟微重力条件下培养小鼠ES细胞。我们使用了不含LIF的无饲养层和无血清培养基。
结论/意义：在此我们表明，模拟微重力允许采用新型的无LIF和无动物源性材料的方法来培养小鼠ES细胞。
