Zhang Junke, Hausmann Ludger, Eibach Rudolf, Welter Leocir J, Töpfer Reinhard, Zyprian Eva M
Julius Kühn Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof, 76833 Siebeldingen, Germany.
Theor Appl Genet. 2009 Oct;119(6):1039-51. doi: 10.1007/s00122-009-1107-1. Epub 2009 Jul 22.
Grapevine rootstock cultivar 'Börner' is a hybrid of Vitis riparia and Vitis cinerea Arnold that shows high resistance to phylloxera (Daktulosphaira vitifoliae Fitch). To localize the determinants of phylloxera root resistance, the susceptible grapevine V3125 (Vitis vinifera 'Schiava grossa' x 'Riesling') was crossed to 'Börner'. Genetic framework maps were built from the progeny. 235 microsatellite markers were placed on the integrated parental map. They cover 1,155.98 cM on 19 linkage groups with an average marker distance of 4.8 cM. Phylloxera resistance was scored by counting nodosities after inoculation of the root system. Progeny plants were triplicated and experimentally infected in 2 years. A scan of the genetic maps indicated a quantitative trait locus on linkage group 13. This region was targeted by six microsatellite-type markers newly developed from the V. vinifera model genome sequence. Two of these appear closely linked to the trait, and can be useful for marker-assisted breeding.
葡萄砧木品种“博纳”是河岸葡萄和灰葡萄阿诺德的杂交品种，对根瘤蚜（葡萄根瘤蚜菲奇）表现出高度抗性。为了定位根瘤蚜抗性的决定因素，将易感葡萄品种V3125（欧亚种“施亚瓦格罗萨”ד雷司令”）与“博纳”杂交。从后代构建了遗传框架图谱。235个微卫星标记被放置在整合的亲本图谱上。它们覆盖了19个连锁群上的1155.98厘摩，平均标记距离为4.8厘摩。通过对接种根系后的瘤节计数来评估根瘤蚜抗性。后代植株进行了三次重复，并在两年内进行了实验感染。对遗传图谱的扫描表明在连锁群13上有一个数量性状位点。该区域被从欧亚种模式基因组序列中新开发的六个微卫星型标记靶向。其中两个标记似乎与该性状紧密连锁，可用于标记辅助育种。
