Holtgräwe Daniela, Rosleff Soerensen Thomas, Hausmann Ludger, Pucker Boas, Viehöver Prisca, Töpfer Reinhard, Weisshaar Bernd
Faculty of Biology, Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
Institute for Grapevine Breeding Geilweilerhof, Julius Kuehn-Institute, Federal Research Centre for Cultivated Plants, Siebeldingen, Germany.
Front Plant Sci. 2020 Mar 4;11:156. doi: 10.3389/fpls.2020.00156. eCollection 2020.
Grapevine breeding has become highly relevant due to upcoming challenges like climate change, a decrease in the number of available fungicides, increasing public concern about plant protection, and the demand for a sustainable production. Downy mildew caused by is one of the most devastating diseases worldwide of cultivated . In modern breeding programs, therefore, genetic marker technologies and genomic data are used to develop new cultivars with defined and stacked resistance loci. Potential sources of resistance are wild species of American or Asian origin. The interspecific hybrid of Gm 183 x Arnold, available as the rootstock cultivar 'Börner,' carries several relevant resistance loci. We applied next-generation sequencing to enable the reliable identification of simple sequence repeats (SSR), and we also generated a draft genome sequence assembly of 'Börner' to access genome-wide sequence variations in a comprehensive and highly reliable way. These data were used to cover the 'Börner' genome with genetic marker positions. A subset of these marker positions was used for targeted mapping of the resistance locus, , to validate the marker position list. Based on the reference genome sequence PN40024, the position of this resistance locus can be narrowed down to less than 0.5 Mbp on chromosome 5.
由于气候变化、可用杀菌剂数量减少、公众对植物保护的关注度不断提高以及对可持续生产的需求等即将到来的挑战，葡萄育种变得高度相关。由[病原菌名称未给出]引起的霜霉病是全球栽培[葡萄品种未给出]最具毁灭性的病害之一。因此，在现代育种计划中，遗传标记技术和基因组数据被用于培育具有明确和叠加抗性基因座的新品种。潜在的抗性来源是美洲或亚洲起源的野生种。美洲葡萄Gm 183与[品种名称未给出]阿诺德的种间杂种，作为砧木品种“博纳”可用，携带几个相关的抗性基因座。我们应用下一代测序技术来可靠地鉴定简单序列重复（SSR），并且我们还生成了“博纳”的基因组序列草图组装，以全面且高度可靠的方式获取全基因组序列变异。这些数据被用于用遗传标记位置覆盖“博纳”基因组。这些标记位置的一个子集被用于对霜霉病抗性基因座[基因座名称未给出]进行靶向定位，以验证标记位置列表。基于参考基因组序列PN40024，该抗性基因座的位置可缩小到第5号染色体上小于0.5兆碱基对的范围。
