葡萄微卫星重复序列作为序列标记位点 (STSs) 分析时可揭示 DNA 多态性。
Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs).
机构信息
CSIRO Division of Horticulture, GPO Box 350, 5001, Adelaide, SA, Australia.
出版信息
Theor Appl Genet. 1993 Sep;86(8):985-90. doi: 10.1007/BF00211051.
Microsatellite repeat sequences were investigated as sequenced-tagged site (STS) DNA markers to determine the potential for genetic analysis of the grapevine genome. The PCR-generated markers detect codominant alleles at a single locus or site in the genome. The marker type is very informative detecting high heterozygosity (69%-88%) within individual grapevine cultivars and high genetic variation between cultivars, making it a useful marker type for plant genome mapping and genome typing. For five loci a screening of 26 V. vinifera cultivars found 13, 12, 8, 5, and 4 different length alleles respectively with some alleles more common than others. The genomic DNA sequences surrounding microsatellite sequences were conserved within the genus permitting STS primers to amplify STSs from other Vitis species. These Vitis species were found to have some unique alleles not present in V. vinifera.
微卫星重复序列被用作序列标记位点（STS）DNA 标记，以确定对葡萄基因组进行遗传分析的潜力。PCR 产生的标记在基因组中的单个基因座或位点检测显性等位基因。该标记类型信息量非常大，在单个葡萄品种内检测到高杂合性（69%-88%），在品种间检测到高遗传变异，使其成为植物基因组作图和基因组分型的有用标记类型。在对 26 个 V.vinifera 品种的五个基因座进行筛选时，发现分别有 13、12、8、5 和 4 个不同长度的等位基因，其中一些等位基因比其他等位基因更常见。微卫星序列周围的基因组 DNA 序列在属内是保守的，允许 STS 引物从其他葡萄物种中扩增 STS。这些葡萄物种被发现具有一些在 V.vinifera 中不存在的独特等位基因。
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