通过纸片扩散诱导试验检测葡萄球菌中诱导性克林霉素耐药性
Detection of inducible clindamycin resistance in staphylococci by disk diffusion induction test.
作者信息
Chelae Sureerat, Laohaprertthisarn Varaporn, Phengmak Manthana, Kongmuang Urairat, Kalnauwakul Sineenart
机构信息
Microbiology Unit, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
出版信息
J Med Assoc Thai. 2009 Jul;92(7):947-51.
OBJECTIVE
To detect inducible clindamycin (CL) resistance in staphylococci by disk diffusion induction test (D-test).
MATERIAL AND METHOD
One thousand one hundred eighty clinical isolates of staphylococci were tested for inducible CL resistance by placing erythromycin (E) disk and clindamycin disk 12 mm apart (edge to edge) on Mueller-Hinton agar plate inoculated with staphylococci. The flattening of CL zone (D-shaped zone) near E disk indicated an inducible CL resistance was observed after 18-24 h of incubation.
RESULTS
Inducible CL resistance was detected in 9.9% of staphylococci isolates. It was found in methicillin-resistant Staphylococcus aureus (MRSA) more than methicillin-sensitive Staphylococcus aureus (MSSA) and coagulase-negative staphylococci (CoNS) 35.9%, 4.7%, and 5.5%, respectively.
CONCLUSION
To avoid misinterpretation of CL result, D-test is recommended for routine detecting of inducible CL resistance in staphylococci. It provides the confident laboratory report of CL as resistant (D-shaped zone positive) or as susceptible (D-shaped zone negative) particular for E resistant isolates.
目的
通过纸片扩散诱导试验（D试验）检测葡萄球菌中诱导性克林霉素（CL）耐药性。
材料与方法
将红霉素（E）纸片和克林霉素纸片在接种了葡萄球菌的穆勒-欣顿琼脂平板上边缘对边缘放置12毫米，对1180株临床分离的葡萄球菌进行诱导性CL耐药性检测。孵育18 - 24小时后，若克林霉素纸片抑菌圈（D形抑菌圈）在靠近红霉素纸片处变平，则表明存在诱导性CL耐药。
结果
在9.9%的葡萄球菌分离株中检测到诱导性CL耐药。在耐甲氧西林金黄色葡萄球菌（MRSA）中发现的比例高于甲氧西林敏感金黄色葡萄球菌（MSSA）和凝固酶阴性葡萄球菌（CoNS），分别为35.9%、4.7%和5.5%。
结论
为避免对克林霉素结果的误判，建议采用D试验常规检测葡萄球菌中的诱导性CL耐药。对于红霉素耐药菌株，它能提供可靠的实验室报告，表明克林霉素耐药（D形抑菌圈阳性）或敏感（D形抑菌圈阴性）。
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