Sayed S M, Abou El-Ella Ghada A, Wahba Nahed M, El Nisr Neveen A, Raddad Khaled, Abd El Rahman M F, Abd El Hafeez M M, Abd El Fattah Aamer Ahmed
Animal Health Research, Cairo, Egypt.
J Med Food. 2009 Jun;12(3):569-75. doi: 10.1089/jmf.2008.0171.
The objective of this work was to evaluate the potency of bee product-immunized rats to overcome an induced Staphylococcus aureus infection. Forty rats were divided to eight groups: T1, T3, and T5 received, respectively, fennel honey, ethanol, and aqueous propolis extracts orally, and T2, T4, and T6 were administered the respective materials intraperitoneally; T7 received bee venom by the bee sting technique; and T8 was the control group. All groups were challenged by a bovine clinical mastitis isolate of S. aureus. Each rat received 2 mL of broth inoculated with 1 x 10(5) colony-forming units/mL intraperitoneally. Two weeks post-induced infection all rats were sacrificed and eviscerated for postmortem inspection and histopathological study. Three rats from T8 and one rat from T7 died before sacrifice. Another two rats, one each in T4 and T5, had morbidity manifestations. The remaining experimental animals showed apparently healthy conditions until time of sacrifice. Postmortem inspection revealed that all T8 rats showed different degrees of skeletal muscle and internal organ paleness with scattered focal pus nodules mainly on lungs and livers. All rats of the treated groups showed normal postmortem features except three rats. A dead rat in group T7 showed focal pus nodules on the lung surface only, whereas the affected two rats in groups T4 and T5 appeared normal except with some pus nodules, but much smaller than in the control, scattered on the hepatic surface and mesentery. Histopathological studies revealed that T8 rats had typical suppurative bronchopneumonia and or severe degenerative and necrobiotic changes in hepatic tissues. Three affected rats of the treated groups showed slight bronchopneumonia or degenerative hepatic changes only. The other animals of the treated groups showed completely normal parenchymatous organs with stimulated lymphatic tissues. It was concluded that all tested previously bee product-immunized rats could significantly challenge the induced S. aureus infection (P < .01). The effects were more pronounced in rats that had received fennel honey solution.
这项工作的目的是评估经蜂产品免疫的大鼠克服诱导性金黄色葡萄球菌感染的能力。40只大鼠被分为8组：T1、T3和T5组分别口服茴香蜂蜜、乙醇和蜂胶水提取物，T2、T4和T6组分别腹腔注射相应物质；T7组通过蜂蛰技术接受蜂毒；T8组为对照组。所有组均受到一株牛临床乳腺炎分离的金黄色葡萄球菌的攻击。每只大鼠腹腔注射2 mL接种有1×10⁵菌落形成单位/ mL的肉汤。诱导感染两周后，所有大鼠被处死并解剖进行尸检和组织病理学研究。T8组的3只大鼠和T7组的1只大鼠在处死前死亡。另外两只大鼠，T4组和T5组各有1只，出现发病表现。其余实验动物在处死前均表现出明显健康的状态。尸检显示，所有T8组大鼠均表现出不同程度的骨骼肌和内脏苍白，主要在肺和肝脏上有散在的局灶性脓结节。除3只大鼠外，所有治疗组的大鼠尸检特征均正常。T7组的1只死亡大鼠仅在肺表面有局灶性脓结节，而T4组和T5组受影响的2只大鼠除肝表面和肠系膜上有一些脓结节外，外观正常，但比对照组小得多。组织病理学研究显示，T8组大鼠有典型的化脓性支气管肺炎和/或肝组织严重的退行性和坏死性变化。治疗组的3只受影响大鼠仅表现出轻微的支气管肺炎或肝退行性变化。治疗组的其他动物实质器官完全正常，淋巴组织有增生。结论是，所有先前经蜂产品免疫的受试大鼠都能显著抵抗诱导性金黄色葡萄球菌感染（P <.01）。在接受茴香蜂蜜溶液的大鼠中效果更明显。
