Kubota M, Izumida A, Takuma H, Kodama K, Sasaki N
Division of Veterinary Microbiology, Kyoto Biken Laboratories, Inc., Japan.
Nihon Juigaku Zasshi. 1990 Dec;52(6):1229-35. doi: 10.1292/jvms1939.52.1229.
Colostrum-deprived, neonatal, 2 days old pigs were inoculated with the attenuated HT-/SK or the virulent 90HS strain of porcine parvovirus (PPV) by the oral or subcutaneous route and sacrificed 2, 4 or 6 days after inoculation. Then, comparison was made on viral multiplication in pigs between the two strains. Pigs inoculated with the HT-/SK strain showed no detectable viremia or HI antibody responses against PPV within 6 days after inoculation. Only in pigs inoculated by the subcutaneous route, a small amount of virus was recovered from the spleen, liver, or mesenteric lymph nodes. These viruses were distinguished from the parental virulent 90HS strain, as examined for rct maker in vitro. When pigs were inoculated with the virulent 90HS strain, viremia appeared in all of them 1 day after inoculation and continued for up to the sacrificed day. Moreover, a considerable amount of virus was also detected from all tissues, including brain, lung, liver, spleen, pancreas, small intestine, and lymph node tissues, in all pigs tested. HI antibodies were first detected 6 days after inoculation.
将初乳剥夺的2日龄新生仔猪通过口服或皮下途径接种猪细小病毒（PPV）的减毒HT-/SK株或强毒90HS株，并在接种后2、4或6天处死。然后，对两株病毒在猪体内的增殖情况进行比较。接种HT-/SK株的仔猪在接种后6天内未检测到病毒血症或针对PPV的血凝抑制（HI）抗体反应。仅通过皮下途径接种的仔猪，在脾脏、肝脏或肠系膜淋巴结中回收了少量病毒。经体外rct标记物检测，这些病毒与亲本强毒90HS株不同。当仔猪接种强毒90HS株时，接种后1天所有仔猪均出现病毒血症，并持续至处死日。此外，在所有检测的仔猪中，从包括脑、肺、肝、脾、胰腺、小肠和淋巴结组织在内的所有组织中也检测到了大量病毒。接种后6天首次检测到HI抗体。
