Suppr超能文献

轮状病毒在初乳喂养和初乳缺乏仔猪中的复制

Rotavirus replication in colostrum-fed and colostrum-deprived pigs.

作者信息

Shaw D P, Morehouse L G, Solorzano R F

机构信息

Department of Veterinary Pathology, College of Veterinary Medicine, University of Missouri, Columbia 65211.

出版信息

Am J Vet Res. 1989 Nov;50(11):1966-70.

PMID:2559636
Abstract

A porcine rotavirus isolate was titrated in neonatal colostrum-fed and colostrum-deprived pigs. The stock rotavirus suspension had a titer of 10(-6.5)/ml and was in its fifteenth cell culture passage in MA-104 cells. Fourteen colostrum-fed pigs were orally inoculated with dilutions of the stock virus suspension ranging from undiluted to 10(-5). These pigs did not develop notable clinical signs during the 7-day experimental trial and no pathologic changes were found in intestine, liver, lung, kidney, spleen, or brain. However, rotavirus was detected in feces of the colostrum-fed pigs, using virus isolation and electron microscopic techniques. Rotavirus was also isolated from lung, brain, or spleen of 4 of 12 of these pigs. Sixteen colostrum-deprived pigs were orally inoculated with dilutions of the stock virus suspension ranging from 10(-1) to 10(-8). Diarrhea developed in 10 of 12 pigs that were given up to the 10(-6) dilution. Seven of these 12 pigs died because of the severity of diarrhea. Pigs that died of rotavirus-induced diarrhea had severe villus loss in the jejunum and ileum. Villi of the small intestine of colostrum-deprived pigs that survived the severe diarrhea were within normal limits at the end of the 7-day trial. The colostrum-deprived pigs that were inoculated with a dilution less than 10(-6) and survived past 96 hours underwent seroconversion. Rotavirus was detected by virus isolation and electron microscopy in the feces of all colostrum-deprived pigs that survived beyond 18.5 hours after inoculation. Virus was isolated from lungs, brain, or spleen of 12 of 16 colostrum-deprived pigs.

摘要

一株猪轮状病毒分离株在初乳喂养和未喂初乳的新生仔猪中进行了滴定。轮状病毒储备悬液的滴度为10^(-6.5)/ml,处于其在MA-104细胞中的第15代细胞培养传代。14头初乳喂养的仔猪经口接种了从原液到10^(-5)稀释度的储备病毒悬液。在为期7天的实验期间,这些仔猪未出现明显的临床症状,在肠道、肝脏、肺、肾脏、脾脏或大脑中未发现病理变化。然而,使用病毒分离和电子显微镜技术在初乳喂养仔猪的粪便中检测到了轮状病毒。在这些仔猪中的12头中的4头的肺、脑或脾脏中也分离到了轮状病毒。16头未喂初乳的仔猪经口接种了从10^(-1)到10^(-8)稀释度的储备病毒悬液。在给予高达10^(-6)稀释度的12头仔猪中,有10头发病腹泻。这12头仔猪中有7头因腹泻严重而死亡。死于轮状病毒引起腹泻的仔猪空肠和回肠绒毛严重缺失。在为期7天的试验结束时,在严重腹泻中存活下来的未喂初乳仔猪的小肠绒毛在正常范围内。接种稀释度小于10^(-6)且存活超过96小时的未喂初乳仔猪发生了血清转化。在接种后存活超过18.5小时的所有未喂初乳仔猪的粪便中,通过病毒分离和电子显微镜检测到了轮状病毒。在16头未喂初乳的仔猪中的12头的肺、脑或脾脏中分离到了病毒。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验