URMITE, CNRS IRD UMR 6236, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
Int J Syst Evol Microbiol. 2009 Dec;59(Pt 12):2956-61. doi: 10.1099/ijs.0.002865-0. Epub 2009 Jul 23.
A total of 11 Bartonella isolates were recovered from the blood of Melomys, Uromys and Rattus species in Australia and were characterized using phenotypic and genotypic methods. Comparison of 16S rRNA gene, ftsZ, gltA and 16S-23S rRNA internal transcribed spacer region sequences from the isolates indicated that they formed three sequence similarity groups that were distinct from one another and from the currently recognized Bartonella species. Phylogenetic analysis based on alignment of concatenated sequences inferred distinct evolutionary lineages for each of the three groups within the genus Bartonella. On the basis of these data, we propose the isolates be accommodated in three novel Bartonella species, namely Bartonella rattaustraliani sp. nov. (type strain AUST/NH4(T) =CIP 109051(T) =CCUG 52161(T) =CSUR B609(T)), Bartonella queenslandensis sp. nov. (type strain AUST/NH12(T) =CIP 109057(T) =CCUG 52167(T) =CSUR B617(T)) and Bartonella coopersplainsensis sp. nov. (type strain AUST/NH20(T) =CIP 109064(T) =CCUG 52174(T) =CSUR B619(T)).
从澳大利亚的 Melomys、Uromys 和 Rattus 物种的血液中总共分离出 11 株 Bartonella 分离株，并用表型和基因型方法进行了特征描述。对分离株的 16S rRNA 基因、ftsZ、gltA 和 16S-23S rRNA 内部转录间隔区序列进行比较表明，它们形成了三个彼此不同且与目前公认的 Bartonella 物种不同的序列相似性组。基于串联序列比对的系统发育分析推断出，在 Bartonella 属内的三个组内，每个组都有独特的进化谱系。根据这些数据，我们建议将这些分离株归入三个新的 Bartonella 种，即 Bartonella rattaustraliani sp. nov.（模式株 AUST/NH4(T)=CIP 109051(T)=CCUG 52161(T)=CSUR B609(T)）、Bartonella queenslandensis sp. nov.（模式株 AUST/NH12(T)=CIP 109057(T)=CCUG 52167(T)=CSUR B617(T)）和 Bartonella coopersplainsensis sp. nov.（模式株 AUST/NH20(T)=CIP 109064(T)=CCUG 52174(T)=CSUR B619(T)）。
