Divari Sara, Pregel Paola, Zanet Stefania, Ferroglio Ezio, Giannini Francesca, Scaglione Frine Eleonora, Grinberg Alex, Biolatti Bartolomeo, Bollo Enrico
Dipartimento di Scienze Veterinarie, Università degli Studi di Torino, 10095 Grugliasco (TO), Italy.
Parco Nazionale Arcipelago Toscano, 57037 Portoferraio (LI), Italy.
Animals (Basel). 2020 Nov 9;10(11):2070. doi: 10.3390/ani10112070.
Wild rodents are reservoirs of several species that cause human bartonellosis. The aim of this study was to assess the presence of spp. DNA in wild rodents in Pianosa island, Italy. Rats ( spp.; 15) and field mice ( spp.; 16) were captured and spleen DNA tested for the presence of spp. by means of an initial screening using a qPCR amplifying a short segment of the 16S-23S rRNA gene intergenic transcribed spacer region (ITS, 200 bp) followed by conventional PCR amplification of a longer ITS fragment (600 bp) and of a citrate synthase (, ~340 bp) gene segment. A total of 25 spleen DNA samples obtained from 31 rodent carcasses (81%) yielded positive qPCR results. genus was confirmed by amplicon sequencing. By conventional PCR, eight out of 25 samples (32%) yielded bands on gels consistent with ITS segment, and 6/25 (24%) yielded bands consistent with the locus. Amplicon sequencing identified and in 1/25 (4%), and 4/25 (16%) samples, respectively. Moreover, 5/25 (20%) of spp. positive samples showed sequences with about 97% identity to . These results provide support to recently published observations suggesting that circulates in wild rodent populations.
野生啮齿动物是多种可导致人类巴尔通体病的病原体的宿主。本研究旨在评估意大利皮亚诺萨岛野生啮齿动物中巴尔通体属物种DNA的存在情况。捕获了大鼠（鼠属；15只）和田鼠（小鼠属；16只），并对其脾脏DNA进行检测，通过最初使用qPCR扩增16S - 23S rRNA基因间隔转录区（ITS，约200 bp）的短片段进行筛选，随后对较长的ITS片段（约600 bp）和柠檬酸合酶（gltA，约340 bp）基因片段进行常规PCR扩增，以检测巴尔通体属物种DNA的存在。从31具啮齿动物尸体中获取的25份脾脏DNA样本（81%）qPCR结果呈阳性。通过扩增子测序确认了巴尔通体属。通过常规PCR，25份样本中有8份（32%）在凝胶上产生了与ITS片段一致的条带，6/25（24%）产生了与gltA位点一致的条带。扩增子测序分别在1/25（4%）和4/25（16%）的样本中鉴定出了汉赛巴尔通体和五日热巴尔通体。此外，25份巴尔通体属阳性样本中有5份（20%）显示出与伊丽莎白巴尔通体序列具有约97%同一性的序列。这些结果为最近发表的表明巴尔通体在野生啮齿动物种群中传播的观察结果提供了支持。
