Yoon Jung-Hoon, Kang So-Jung, Oh Ki-Hoon, Oh Tae-Kwang
Korea Research Institute of Bioscience and Biotechnology, Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2839-42. doi: 10.1099/ijs.0.010215-0. Epub 2009 Jul 23.
A Gram-variable-staining, motile and coccoid-, ovoid- or rod-shaped bacterium, strain ISL-25(T), was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain ISL-25(T) grew optimally at pH 7.0-8.0 and 30-37 degrees C. Strain ISL-25(T) contained meso-diaminopimelic acid as the cell-wall peptidoglycan, MK-7 as the predominant menaquinone and anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) as the major fatty acids. The DNA G+C content was 49.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ISL-25(T) belongs to the genus Salimicrobium. The similarity values between the 16S rRNA gene sequence of strain ISL-25(T) and those of the type strains of the three currently recognized Salimicrobium species were 97.6-98.3 %. Mean DNA-DNA relatedness values between strain ISL-25(T) and the type strains of the genus Salimicrobium were 9-15 %. Differential phenotypic properties of strain ISL-25(T), together with the phylogenetic and genetic distinctiveness, revealed that this strain could be differentiated from other Salimicrobium species. Therefore, strain ISL-25(T) represents a novel species within the genus Salimicrobium, for which the name Salimicrobium flavidum sp. nov. is proposed. The type strain is ISL-25(T) (=KCTC 13260(T)=CCUG 56755(T)).
从韩国黄海的一个海洋太阳能盐场分离出一株革兰氏染色可变、运动性且呈球状、卵形或杆状的细菌，菌株ISL-25(T)，并通过多相研究对其分类地位进行了调查。菌株ISL-25(T)在pH 7.0 - 8.0和30 - 37℃下生长最佳。菌株ISL-25(T)含有内消旋二氨基庚二酸作为细胞壁肽聚糖，MK-7作为主要的甲基萘醌，anteiso-C(15 : 0)、anteiso-C(17 : 0)和iso-C(16 : 0)作为主要脂肪酸。DNA G + C含量为49.3 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株ISL-25(T)属于盐微菌属。菌株ISL-25(T)的16S rRNA基因序列与目前认可的三个盐微菌属种的模式菌株的相似性值为97.6 - 98.3%。菌株ISL-25(T)与盐微菌属模式菌株之间的平均DNA - DNA相关性值为9 - 15%。菌株ISL-25(T)的不同表型特征，连同系统发育和遗传独特性，表明该菌株可与其他盐微菌属种区分开来。因此，菌株ISL-25(T)代表盐微菌属内的一个新种，为此提出新名称黄色盐微菌（Salimicrobium flavidum sp. nov.）。模式菌株为ISL-25(T)（=KCTC 13260(T)=CCUG 56755(T)）。
