Yoon Jung-Hoon, Kang So-Jung, Jung Yong-Taek, Oh Tae-Kwang
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2404-7. doi: 10.1099/ijs.0.008359-0. Epub 2009 Jul 20.
A Gram-negative, non-motile and rod-shaped bacterial strain, ISL-14(T), was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by a polyphasic study. Strain ISL-14(T) grew optimally at pH 7.0-8.0, 30 degrees C and in the presence of approximately 2 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone and anteiso-C(15 : 0), iso-C(15 : 0) and iso-C(16 : 0) 3-OH as the major fatty acids. The DNA G+C content was 38.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-14(T) belonged to the genus Psychroflexus. The levels of similarity between the 16S rRNA gene sequence of strain ISL-14(T) and those of the type strains of recognized Psychroflexus species were 95.8-96.8 %. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain ISL-14(T) represents a novel species within the genus Psychroflexus, for which the name Psychroflexus salinarum sp. nov. is proposed. The type strain is ISL-14(T) (=KCTC 22483(T)=CCUG 56752(T)).
从韩国黄海的一个海洋太阳能盐场中分离出一株革兰氏阴性、无运动性的杆状细菌菌株ISL-14(T)，并通过多相研究对其分类地位进行了调查。菌株ISL-14(T)在pH 7.0-8.0、30℃以及约2%（w/v）NaCl存在的条件下生长最佳。它以MK-6作为主要的甲基萘醌，以anteiso-C(15 : 0)、iso-C(15 : 0)和iso-C(16 : 0) 3-OH作为主要脂肪酸。DNA G+C含量为38.5 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株ISL-14(T)属于嗜冷弯曲菌属。菌株ISL-14(T)的16S rRNA基因序列与已确认的嗜冷弯曲菌属模式菌株的序列相似性水平为95.8-96.8%。基于表型、化学分类和系统发育数据，菌株ISL-14(T)代表嗜冷弯曲菌属内的一个新物种，为此提出了新名称嗜盐嗜冷弯曲菌（Psychroflexus salinarum sp. nov.）。模式菌株为ISL-14(T)（=KCTC 22483(T)=CCUG 56752(T)）。
