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Expression of human immunodeficiency virus type 1 gag gene using genetically engineered herpes simplex virus type 1 recombinants.

作者信息

Rösen-Wolff A, Raab K, Zöller L, Darai G, Eberle J, Deinhardt F

机构信息

Institut für Medizinische Virologie der Universität Heidelberg, FRG.

出版信息

Virus Genes. 1990 Dec;4(4):325-37. doi: 10.1007/BF00570027.

DOI:10.1007/BF00570027
PMID:1962977
Abstract

Infectious herpes simplex virus type 1 (HSV-1) recombinants were constructed by inserting the cDNA sequence of the human immunodeficiency virus type 1 (HIV-1) gag gene (from nucleotide position 675 [SacI] to 3859 [Asp718] of the cDNA sequences of HIV-1 strain BH-10) within the DNA sequences of the BamHI DNA fragment B of the genome of an apathogenic HSV-1 strain HFEM. This HSV-1 strain possesses a 4.1-kbp deletion within the BamHI DNA fragment B between 0.762 and 0.789 map units of the viral genome, which allows the insertion of at least 4 kbp of foreign genetic material into this particular region. The DNA sequences of the immediate early promoter (IE4) of HSV-1 that were inserted upstream from the gag gene were used as a promoter. The screening of 205 virus stocks derived from individual plaques revealed that 46 recombinant viruses harbor HIV-1 gag-specific DNA sequences. However, it was found that only six of the recombinant viruses are able to express the gag gene product of HIV-1. This indicates that the ratio of the positive recombination events is about 2.9%.

摘要

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本文引用的文献

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Induction of generalized and lethal herpesvirus infection in the tree shrew by intrahepatic transfection of herpes simplex virus DNA.
J Virol Methods. 1983 Dec;7(5-6):305-14. doi: 10.1016/0166-0934(83)90083-6.
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The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
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DNA sequence analysis of an immediate-early gene region of the herpes simplex virus type 1 genome (map coordinates 0.950 to 0.978).单纯疱疹病毒1型基因组即刻早期基因区域（图谱坐标0.950至0.978）的DNA序列分析
J Gen Virol. 1982 Sep;62 (Pt 1):1-15. doi: 10.1099/0022-1317-62-1-1.
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DNA typing from single hairs.单根毛发的DNA分型
Nature. 1988 Apr 7;332(6164):543-6. doi: 10.1038/332543a0.
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Determination of the nucleotide sequence flanking the deletion (0.762 to 0.789 map units) in the genome of an intraperitoneally avirulent HSV-1 strain HFEM.对腹腔无毒力的单纯疱疹病毒1型（HSV-1）HFEM株基因组中缺失区域（0.762至0.789个图距单位）侧翼核苷酸序列的测定。
Virus Res. 1987 Apr;7(2):105-15. doi: 10.1016/0168-1702(87)90073-6.
10
Replacement of the deletion in the genome (0.762-0.789 mu) of avirulent HSV-1 HFEM using cloned MluI DNA fragment (0.7615-0.796 mu) of virulent HSV-1 F leads to generation of virulent intratypic recombinant.使用强毒株单纯疱疹病毒1型（HSV-1）F的克隆MluI DNA片段（0.7615 - 0.796微米）替换无毒株HSV-1 HFEM基因组中的缺失片段（0.762 - 0.789微米），会导致产生强毒株型内重组体。
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