Liu E-Hu, Qi Lian-Wen, Cheng Xiao-Lan, Peng Yong-bo, Li Ping
Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Ministry of Education; Nanjing, 210009, People's Republic of China.
Biomed Chromatogr. 2010 Feb;24(2):125-31. doi: 10.1002/bmc.1269.
Buyang Huanwu Decoction (BYHYD) is a classical traditional Chinese medicinal prescription that has been widely used for treating cerebrovascular illnesses for hundreds of years. In this study, a comprehensive analytical method has been developed for quantitative analysis of the major constituents in BYHWD. This method was based on high-performance liquid chromatography coupled to a diode array and evaporative light scattering detectors (HPLC-DAD-ELSD) on a common reverse-phase C(18) column. HPLC coupled with on-line time-of-flight mass spectrometry (HPLC-TOF/MS) was additionally adopted to provide further validation for the constituents. It was found that 0.3% aqueous formic acid and acetonitrile was the optimum mobile phase for gradient elution. This method, which showed excellent precision and accuracy, was successfully applied to quantify the bioactive constituents in six BYHWD products. The validated HPLC-DAD-ELSD method, together with the HPLC-TOF/MS analysis, provided a new basis for assessing the quality of traditional Chinese medicinal prescription consisting of many bioactive components.
补阳还五汤是一个经典的中药方剂，数百年来一直广泛用于治疗脑血管疾病。在本研究中，已开发出一种综合分析方法用于补阳还五汤中主要成分的定量分析。该方法基于在普通反相C(18)柱上的高效液相色谱联用二极管阵列和蒸发光散射检测器（HPLC-DAD-ELSD）。另外采用HPLC与在线飞行时间质谱联用（HPLC-TOF/MS）为成分提供进一步验证。发现0.3%甲酸水溶液和乙腈是梯度洗脱的最佳流动相。该方法具有出色的精密度和准确度，已成功应用于六种补阳还五汤产品中生物活性成分的定量。经过验证的HPLC-DAD-ELSD方法以及HPLC-TOF/MS分析为评估由多种生物活性成分组成的中药方剂质量提供了新依据。
